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3.A.1.25.4
The biotin uptake system, BioMNY. The 3-d structure of the EcfS subunit, BioY, at 2.1Å resolution is known (Berntsson et al., 2012). BioY and ThiT from L. lactis show similar N-terminal structures for interaction with the ECF module but divergent C-terminal structures for substrate binding. BioY alone binds biotin but doesn''t transport it (Berntsson et al., 2012).  Ala-Arg-Ser and Ala-Arg-Gly signatures in BioN are probably coupling sites to the two BioM ATPase subunits (Neubauer et al., 2011Neubauer et al., 2011).

Accession Number:A2RI01
Protein Name:Energy-coupling factor transporter ATP-binding protein EcfA 1
Length:277
Molecular Weight:30931.00
Species:Lactococcus lactis subsp. cremoris (strain MG1363) [416870]
Location1 / Topology2 / Orientation3: Cell membrane1 / Peripheral membrane protein2
Substrate Biotin

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FASTA formatted sequence
1:	MNKILEVENL VFKYEKESDV NQLNGVSFSI TKGEWVSIIG QNGSGKSTTA RLIDGLFEEF 
61:	EGIVKIDGER LTAENVWNLR RKIGMVFQNP DNQFVGATVE DDVAFGMENQ GIPREEMIKR 
121:	VDEALLAVNM LDFKTREPAR LSGGQKQRVA VAGIIALRPE IIILDESTSM LDPTGRSEIM 
181:	RVIHEIKDKY HLTVLSITHD LDEAASSDRI LVMRAGEIIK EAAPSELFAT SEDMVEIGLD 
241:	VPFSSNLMKD LRTNGFDLPE KYLSEDELVE LLADKLG