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3.A.1.4.1
Leucine; leucine/isoleucine/valine porter (also transports phenylalanine and tyrosine; Koyanagi et al., 2004)

Accession Number:P04816
Protein Name:Livk aka B3458
Length:369
Molecular Weight:39379.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Periplasm1
Substrate phenylalanine, Valine, Isoleucine, Leucine, tyrosine

Cross database links:

Genevestigator: P04816
EchoBASE: EB0535
EcoGene: EG10540
eggNOG: COG0683
HEGENOM: HBG695960
RefSeq: AP_004334.1    NP_417915.1   
Entrez Gene ID: 947964   
Pfam: PF01094   
BioCyc: EcoCyc:LIVK-MONOMER    ECOL168927:B3458-MONOMER   
KEGG: ecj:JW3423    eco:b3458   

Gene Ontology

GO:0030288 C:outer membrane-bounded periplasmic space
GO:0006865 P:amino acid transport

References (9)

[1] “The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport.”  Landick R.et.al.   3891753
[2] “The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function.”  Antonucci T.K.et.al.   4077929
[3] “Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli.”  Adams M.D.et.al.   2195019
[4] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[5] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[6] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[7] “Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form.”  Oxender D.L.et.al.   6990419
[8] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link A.J.et.al.   9298646
[9] “Structure of the L-leucine-binding protein refined at 2.4-A resolution and comparison with the Leu/Ile/Val-binding protein structure.”  Sack J.S.et.al.   2649683
Structure:
1USG   1USI   1USK   2LBP     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKRNAKTIIA GMIALAISHT AMADDIKVAV VGAMSGPIAQ WGDMEFNGAR QAIKDINAKG 
61:	GIKGDKLVGV EYDDACDPKQ AVAVANKIVN DGIKYVIGHL CSSSTQPASD IYEDEGILMI 
121:	SPGATNPELT QRGYQHIMRT AGLDSSQGPT AAKYILETVK PQRIAIIHDK QQYGEGLARS 
181:	VQDGLKAANA NVVFFDGITA GEKDFSALIA RLKKENIDFV YYGGYYPEMG QMLRQARSVG 
241:	LKTQFMGPEG VGNASLSNIA GDAAEGMLVT MPKRYDQDPA NQGIVDALKA DKKDPSGPYV 
301:	WITYAAVQSL ATALERTGSD EPLALVKDLK ANGANTVIGP LNWDEKGDLK GFDFGVFQWH 
361:	ADGSSTAAK