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3.A.1.4.1
Leucine; leucine/isoleucine/valine porter (also transports phenylalanine and tyrosine; Koyanagi et al., 2004)

Accession Number:P0AD96
Protein Name:Leu/Ile/Val-binding protein LivJ aka B3460 aka LIV-BP
Length:367
Molecular Weight:39076.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Periplasm1
Substrate phenylalanine, Valine, Isoleucine, Leucine, tyrosine

Cross database links:

Genevestigator: P0AD96
EchoBASE: EB0534
EcoGene: EG10539
eggNOG: COG0683
HEGENOM: HBG695960
RefSeq: AP_004332.1    YP_026223.1   
Entrez Gene ID: 947971   
Pfam: PF01094   
BioCyc: EcoCyc:LIVJ-MONOMER   
KEGG: ecj:JW3425    eco:b3460   

Gene Ontology

GO:0030288 C:outer membrane-bounded periplasmic space
GO:0006865 P:amino acid transport

References (9)

[1] “The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport.”  Landick R.et.al.   3891753
[2] “The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function.”  Antonucci T.K.et.al.   4077929
[3] “Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli.”  Adams M.D.et.al.   2195019
[4] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[5] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[6] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[7] “The primary structure of a Leu, Ile and Val (LIV)-binding protein from Escherichia coli.”  Ovchinnikov Y.A.et.al.   328304
[8] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link A.J.et.al.   9298646
[9] “Periplasmic binding protein structure and function. Refined X-ray structures of the leucine/isoleucine/valine-binding protein and its complex with leucine.”  Sack J.S.et.al.   2649682
Structure:
1Z15   1Z16   1Z17   1Z18   2LIV     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNIKGKALLA GCIALAFSNM ALAEDIKVAV VGAMSGPVAQ YGDQEFTGAE QAVADINAKG 
61:	GIKGNKLQIV KYDDACDPKQ AVAVANKVVN DGIKYVIGHL CSSSTQPASD IYEDEGILMI 
121:	TPAATAPELT ARGYQLILRT TGLDSDQGPT AAKYILEKVK PQRIAIVHDK QQYGEGLARA 
181:	VQDGLKKGNA NVVFFDGITA GEKDFSTLVA RLKKENIDFV YYGGYHPEMG QILRQARAAG 
241:	LKTQFMGPEG VANVSLSNIA GESAEGLLVT KPKNYDQVPA NKPIVDAIKA KKQDPSGAFV 
301:	WTTYAALQSL QAGLNQSDDP AEIAKYLKAN SVDTVMGPLT WDEKGDLKGF EFGVFDWHAN 
361:	GTATDAK