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3.A.1.3.2
Three component ABC L-glutamine porter. The basal ATPase activity (ATP hydrolysis in the absence of substrate) is mainly caused by the docking of the closed-unliganded state of GlnH onto the transporter domain of GlnPQ. Unlike glutamine, arginine binds both GlnH domains, but does not trigger their closing. Comparison of the ATPase activity in nanodiscs with glutamine transport in proteoliposomes suggested that the stoichiometry of ATP per substrate is close to two (Lycklama A Nijeholt et al. 2018).

Accession Number:P0AEQ6
Protein Name:Glutamine transport system permease protein GlnP aka B0810
Length:219
Molecular Weight:24364.00
Species:Escherichia coli [83333]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Glutamine

Cross database links:

Genevestigator: P0AEQ6
EchoBASE: EB0383
EcoGene: EG10388
eggNOG: COG0765
HEGENOM: HBG704821
DIP: DIP-47993N
RefSeq: AP_001441.1    NP_415331.1   
Entrez Gene ID: 945621   
Pfam: PF00528   
BioCyc: EcoCyc:GLNP-MONOMER    ECOL168927:B0810-MONOMER   
KEGG: ecj:JW0795    eco:b0810   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (5)

[1] “Cloning and complete nucleotide sequence of the Escherichia coli glutamine permease operon (glnHPQ).”  Nohno T.et.al.   3027504
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQFDWSAIWP AIPLLIEGAK MTLWISVLGL AGGLVIGLLA GFARTFGGWI ANHVALVFIE 
61:	VIRGTPIVVQ VMFIYFALPM AFNDLRIDPF TAAVVTIMIN SGAYIAEITR GAVLSIHKGF 
121:	REAGLALGLS RWETIRYVIL PLALRRMLPP LGNQWIISIK DTSLFIVIGV AELTRQGQEI 
181:	IAGNFRALEI WSAVAVFYLI ITLVLSFILR RLERRMKIL