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3.A.1.4.1
Leucine; leucine/isoleucine/valine porter (also transports phenylalanine and tyrosine; Koyanagi et al., 2004)

Accession Number:P0AEX7
Protein Name:High-affinity branched-chain amino acid transport system permease protein LivH aka B3457
Length:308
Molecular Weight:32982.00
Species:Escherichia coli [83333]
Number of TMSs:9
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate phenylalanine, Valine, Isoleucine, Leucine, tyrosine

Cross database links:

Genevestigator: P0AEX7
EchoBASE: EB0533
EcoGene: EG10538
eggNOG: COG0559
HEGENOM: HBG728273
RefSeq: AP_004335.1    NP_417914.1   
Entrez Gene ID: 947965   
Pfam: PF02653   
BioCyc: EcoCyc:LIVH-MONOMER    ECOL168927:B3457-MONOMER   
KEGG: ecj:JW3422    eco:b3457   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006865 P:amino acid transport

References (6)

[1] “Cloning and characterization of livH, the structural gene encoding a component of the leucine transport system in Escherichia coli.”  Nazos P.M.et.al.   3009409
[2] “Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli.”  Adams M.D.et.al.   2195019
[3] “Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.”  Sofia H.J.et.al.   8041620
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSEQFLYFLQ QMFNGVTLGS TYALIAIGYT MVYGIIGMIN FAHGEVYMIG SYVSFMIIAA 
61:	LMMMGIDTGW LLVAAGFVGA IVIASAYGWS IERVAYRPVR NSKRLIALIS AIGMSIFLQN 
121:	YVSLTEGSRD VALPSLFNGQ WVVGHSENFS ASITTMQAVI WIVTFLAMLA LTIFIRYSRM 
181:	GRACRACAED LKMASLLGIN TDRVIALTFV IGAAMAAVAG VLLGQFYGVI NPYIGFMAGM 
241:	KAFTAAVLGG IGSIPGAMIG GLILGIAEAL SSAYLSTEYK DVVSFALLIL VLLVMPTGIL 
301:	GRPEVEKV