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5.A.3.1.1
Anaerobic, respiratory, membrane-bound nitrate reductase, NarGHI. Two protons are consumed in the cytoplasm while two protons are released in the periplasm, contributing to the pmf (Simon et al., 2008).

Accession Number:P11350
Protein Name:NarI aka CHLI aka B1227
Length:225
Molecular Weight:25497.00
Species:Escherichia coli [83333]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Electrons

Cross database links:

Genevestigator: P11350
EchoBASE: EB0634
EcoGene: EG10640
eggNOG: COG2181
HEGENOM: HBG308661
DIP: DIP-10313N
RefSeq: AP_001855.1    NP_415745.1   
Entrez Gene ID: 945808   
Pfam: PF02665   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:NARI-MONOMER    ECOL168927:B1227-MONOMER    MetaCyc:NARI-MONOMER   
KEGG: ecj:JW1218    eco:b1227   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0009325 C:nitrate reductase complex
GO:0005886 C:plasma membrane
GO:0009055 F:electron carrier activity
GO:0005506 F:iron ion binding
GO:0008940 F:nitrate reductase activity
GO:0009061 P:anaerobic respiration
GO:0017004 P:cytochrome complex assembly
GO:0022900 P:electron transport chain
GO:0042128 P:nitrate assimilation
GO:0006810 P:transport

References (7)

[1] “narI region of the Escherichia coli nitrate reductase (nar) operon contains two genes.”  Sodergren E.J.et.al.   2832376
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Roles of the narJ and narI gene products in the expression of nitrate reductase in Escherichia coli.”  Sodergren E.J.et.al.   3053688
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[7] “Insights into the respiratory electron transfer pathway from the structure of nitrate reductase A.”  Bertero M.G.et.al.   12910261
Structure:
1Q16   1SIW   1Y4Z   1Y5I   1Y5L   1Y5N   3EGW   3IR5   3IR6   3IR7   [...more]

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQFLNMFFFD IYPYIAGAVF LIGSWLRYDY GQYTWRAASS QMLDRKGMNL ASNLFHIGIL 
61:	GIFVGHFFGM LTPHWMYEAW LPIEVKQKMA MFAGGASGVL CLIGGVLLLK RRLFSPRVRA 
121:	TTTGADILIL SLLVIQCALG LLTIPFSAQH MDGSEMMKLV GWAQSVVTFH GGASQHLDGV 
181:	AFIFRLHLVL GMTLFLLFPF SRLIHIWSVP VEYLTRKYQL VRARH