TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


3.A.2.2.4
The ubiquitous 14 subunit vacuolar H+-ATPase, V1/V0, has been implicated in various human diseases including osteopetrosis, renal tubule acidosis, and cancer (Hinton et al., 2009).  The transmembrane enzyme, Ribonuclease kappa, RNASEK (137 aas; 2 TMSs; UniProt acc# Q6P5S7), closely associates with the V-ATPase and is required for its function; its loss prevents the early events of endocytosis and the replication of multiple pathogenic viruses (Perreira et al. 2015).

Accession Number:P21281
Protein Name:V-ATPase subunit B2
Length:511
Molecular Weight:56501.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Endomembrane system1 / Peripheral membrane protein2
Substrate H+

Cross database links:

Genevestigator: P21281
eggNOG: prNOG16005
HEGENOM: HBG565875
RefSeq: NP_001684.2   
Entrez Gene ID: 526   
Pfam: PF00006    PF00306    PF02874   
Drugbank: Drugbank Link   
OMIM: 606939  gene
KEGG: hsa:526   

Gene Ontology

GO:0005829 C:cytosol
GO:0012505 C:endomembrane system
GO:0005794 C:Golgi apparatus
GO:0042470 C:melanosome
GO:0005886 C:plasma membrane
GO:0033180 C:proton-transporting V-type ATPase, V1 domain
GO:0046933 F:hydrogen ion transporting ATP synthase acti...
GO:0046961 F:proton-transporting ATPase activity, rotati...
GO:0015986 P:ATP synthesis coupled proton transport

References (8)

[1] “Selectively amplified expression of an isoform of the vacuolar H(+)-ATPase 56-kilodalton subunit in renal intercalated cells.”  Nelson R.D.et.al.   1373501
[2] “Heterogeneity of vacuolar H(+)-ATPase: differential expression of two human subunit B isoforms.”  van Hille B.et.al.   7945239
[3] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Transcriptional regulation of the vacuolar H(+)-ATPase B2 subunit gene in differentiating THP-1 cells.”  Lee B.S.et.al.   7706273
[6] “An mRNA from human brain encodes an isoform of the B subunit of the vacuolar H(+)-ATPase.”  Bernasconi P.et.al.   2145275
[7] “Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.”  Basrur V.et.al.   12643545
[8] “Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.”  Chi A.et.al.   17081065

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MALRAMRGIV NGAAPELPVP TGGPAVGARE QALAVSRNYL SQPRLTYKTV SGVNGPLVIL 
61:	DHVKFPRYAE IVHLTLPDGT KRSGQVLEVS GSKAVVQVFE GTSGIDAKKT SCEFTGDILR 
121:	TPVSEDMLGR VFNGSGKPID RGPVVLAEDF LDIMGQPINP QCRIYPEEMI QTGISAIDGM 
181:	NSIARGQKIP IFSAAGLPHN EIAAQICRQA GLVKKSKDVV DYSEENFAIV FAAMGVNMET 
241:	ARFFKSDFEE NGSMDNVCLF LNLANDPTIE RIITPRLALT TAEFLAYQCE KHVLVILTDM 
301:	SSYAEALREV SAAREEVPGR RGFPGYMYTD LATIYERAGR VEGRNGSITQ IPILTMPNDD 
361:	ITHPIPDLTG YITEGQIYVD RQLHNRQIYP PINVLPSLSR LMKSAIGEGM TRKDHADVSN 
421:	QLYACYAIGK DVQAMKAVVG EEALTSDDLL YLEFLQKFER NFIAQGPYEN RTVFETLDIG 
481:	WQLLRIFPKE MLKRIPQSTL SEFYPRDSAK H