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3.A.1.129.1
Thiol (Cysteine/Glutathione) exporter, CydDC; CydC is also called MdrH (periplasmic cysteine is required for cytochrome bd assembly) (Cruz-Ramos et al., 2004).  The purified asymmetric heterodimer exhibits low ATPase activity which is activated by both thiols and heme (e.g., heme b) compounds, suggesting that heme binds to and activates thiol transport (Yamashita et al. 2014).  Bacterial redox homoeostasis during nitrosative stress is influenced by CydDC.  Periplasmic low molecular weight thiols restore haem incorporation into a cytochrome complex (Holyoake et al. 2016).

Accession Number:P23886
Protein Name:Transport ATP-binding protein CydC
Length:573
Molecular Weight:62920.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate Cysteine, Glutathione

Cross database links:

Genevestigator: P23886
EchoBASE: EB0012
EcoGene: EG10012
eggNOG: COG4987
HEGENOM: HBG758042
DIP: DIP-9362N
RefSeq: AP_001516.1    NP_415406.1   
Entrez Gene ID: 945504   
Pfam: PF00664    PF00005   
BioCyc: EcoCyc:CYDC-MONOMER    ECOL168927:B0886-MONOMER   
KEGG: ecj:JW0869    eco:b0886   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005524 F:ATP binding
GO:0042626 F:ATPase activity, coupled to transmembrane m...
GO:0055085 P:transmembrane transport

References (8)

[1] “The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat.”  Shrader T.E.et.al.   8331068
[2] “Isolation and characterization of an Escherichia coli mutant defective in resuming growth after starvation.”  Siegele D.D.et.al.   8276245
[3] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Cytochrome bd biosynthesis in Escherichia coli: the sequences of the cydC and cydD genes suggest that they encode the components of an ABC membrane transporter.”  Poole R.K.et.al.   7934832
[7] “Structure and expression of the infA operon encoding translational initiation factor IF1. Transcriptional control by growth rate.”  Cummings H.S.et.al.   1909328
[8] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRALLPYLAL YKRHKWMLSL GIVLAIVTLL ASIGLLTLSG WFLSASAVAG VAGLYSFNYM 
61:	LPAAGVRGAA ITRTAGRYFE RLVSHDATFR VLQHLRIYTF SKLLPLSPAG LARYRQGELL 
121:	NRVVADVDTL DHLYLRVISP LVGAFVVIMV VTIGLSFLDF TLAFTLGGIM LLTLFLMPPL 
181:	FYRAGKSTGQ NLTHLRGQYR QQLTAWLQGQ AELTIFGASD RYRTQLENTE IQWLEAQRRQ 
241:	SELTALSQAI MLLIGALAVI LMLWMASGGV GGNAQPGALI ALFVFCALAA FEALAPVTGA 
301:	FQHLGQVIAS AVRISDLTDQ KPEVTFPDTQ TRVADRVSLT LRDVQFTYPE QSQQALKGIS 
361:	LQVNAGEHIA ILGRTGCGKS TLLQQLTRAW DPQQGEILLN DSPIASLNEA ALRQTISVVP 
421:	QRVHLFSATL RDNLLLASPG SSDEALSEIL RRVGLEKLLE DAGLNSWLGE GGRQLSGGEL 
481:	RRLAIARALL HDAPLVLLDE PTEGLDATTE SQILELLAEM MREKTVLMVT HRLRGLSRFQ 
541:	QIIVMDNGQI IEQGTHAELL ARQGRYYQFK QGL