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3.A.2.2.4
The ubiquitous 14 subunit vacuolar H+-ATPase, V1/V0, has been implicated in various human diseases including osteopetrosis, renal tubule acidosis, and cancer (Hinton et al., 2009).  The transmembrane enzyme, Ribonuclease kappa, RNASEK (137 aas; 2 TMSs; UniProt acc# Q6P5S7), closely associates with the V-ATPase and is required for its function; its loss prevents the early events of endocytosis and the replication of multiple pathogenic viruses (Perreira et al. 2015).

Accession Number:P36543
Protein Name:V-ATPase subunit E1
Length:226
Molecular Weight:26145.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Endomembrane system1 / Peripheral membrane protein2
Substrate H+

Cross database links:

Genevestigator: P36543
eggNOG: prNOG13168
HEGENOM: HBG525538
RefSeq: NP_001687.1   
Entrez Gene ID: 529   
Pfam: PF01991   
Drugbank: Drugbank Link   
OMIM: 108746  gene

Gene Ontology

GO:0016324 C:apical plasma membrane
GO:0005829 C:cytosol
GO:0005768 C:endosome
GO:0033178 C:proton-transporting two-sector ATPase compl...
GO:0005515 F:protein binding
GO:0046961 F:proton-transporting ATPase activity, rotati...
GO:0015986 P:ATP synthesis coupled proton transport

References (10)

[1] “Immunologic evidence that vacuolar H+ ATPases with heterogeneous forms of Mr = 31,000 subunit have different membrane distributions in mammalian kidney.”  Hemken P.et.al.   1533641
[2] “The E subunit of vacuolar H(+)-ATPase localizes close to the centromere on human chromosome 22.”  Baud V.et.al.   8004105
[3] “Cloning and tissue distribution of subunits C, D, and E of the human vacuolar H(+)-ATPase.”  van Hille B.et.al.   8250920
[4] “A genome annotation-driven approach to cloning the human ORFeome.”  Collins J.E.et.al.   15461802
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Interaction between aldolase and vacuolar H+-ATPase: evidence for direct coupling of glycolysis to the ATP-hydrolyzing proton pump.”  Lu M.et.al.   11399750
[7] “A human gene, ATP6E1, encoding a testis-specific isoform of H(+)-ATPase subunit E.”  Imai-Senga Y.et.al.   12036578
[8] “Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.”  Rikova K.et.al.   18083107
[9] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[10] “The consensus coding sequences of human breast and colorectal cancers.”  Sjoeblom T.et.al.   16959974

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MALSDADVQK QIKHMMAFIE QEANEKAEEI DAKAEEEFNI EKGRLVQTQR LKIMEYYEKK 
61:	EKQIEQQKKI QMSNLMNQAR LKVLRARDDL ITDLLNEAKQ RLSKVVKDTT RYQVLLDGLV 
121:	LQGLYQLLEP RMIVRCRKQD FPLVKAAVQK AIPMYKIATK NDVDVQIDQE SYLPEDIAGG 
181:	VEIYNGDRKI KVSNTLESRL DLIAQQMMPE VRGALFGANA NRKFLD