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1.A.1.4.6
Heterotetrameric K+ channel, KAT2/AKT2/KCT2 (Nieves-Cordones et al. 2014). Forms heteromeric channels (2:2 stoichiometry) with KAT1 (1.A.1.4.7) (Lebaudy et al., 2010) (Properties differ from those of homomeric channels; Xicluna et al., 2007).  KAT2 also forms homomeric channels in the plasma membrane (Nieves-Cordones et al. 2014). AKT2 functions in phloem loading and unloading and operates as an inward-rectifying channel that allows H+-ATPase-energized K+ uptake. Through reversible post-translational modifications, it can also function as an open, K+-selective channel, providing energy for transmembrane transport processes. It is present in a complex of several proteins in which it interacts with the receptor-like kinase, MRH1/MDIS2 (Sklodowski et al. 2017). The ortholog in Brassica rapa (Chinese cabbage), KCT2, is induced by stress. It has a TxxTxGYGD motif in the P-domain and a putative cyclic nucleotide-binding-like domain within a long C-terminal region (Zhang et al. 2006).  

Accession Number:Q38849
Protein Name:KAT2
Length:708
Molecular Weight:81525.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: Q38849
eggNOG: KOG0498
HEGENOM: HBG318539
RefSeq: NP_193563.3   
Entrez Gene ID: 827555   
Pfam: PF00027    PF11834    PF00520   
KEGG: ath:AT4G18290   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0042802 F:identical protein binding
GO:0005249 F:voltage-gated potassium channel activity
GO:0007623 P:circadian rhythm
GO:0006813 P:potassium ion transport
GO:0009644 P:response to high light intensity
GO:0010118 P:stomatal movement
GO:0055085 P:transmembrane transport

References (3)

[1] “Guard cell inward K+ channel activity in Arabidopsis involves expression of the twin channel subunits KAT1 and KAT2.”  Pilot G.et.al.   11042178
[2] “Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.”  Mayer K.F.X.et.al.   10617198
[3] “Phylogenetic relationships within cation transporter families of Arabidopsis.”  Maeser P.et.al.   11500563

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLKRKHLNTR PMSISCTRNF FKRFCVEEYN MDTFKHSSFL SADLLPSLGA RINQSTKLRK 
61:	HIISPFDPRF RGWEMWLVIL VIYSAWICPF EFAFITYKKD ALFIIDNIVN GFFAIDIILT 
121:	FFVAYLDSHS YLLVDKPKKI AIRYLSTWFA FDVCSTAPFQ SLSLLFKYNG SEIGFRVLSM 
181:	LRLWRLRRVS SLFARLEKDI RFNYFWTRCT KLISVTLFAV HCAGCFAYLI ADQYHDPTKT 
241:	WIGAVYPNFK ETSVWSRYVT ALYWSITTLT TTGYGDLHAE NPREMLFFVF FMLFNLGFTS 
301:	YLIGNMTNLV VHWTSRTRNF RDTVRAASEF ASRNQLPPNI QDQMLSHICL KFKTEGLKQQ 
361:	EALNGLPKAI RSSIANYLFF PIVQNVYLFH GVSRNFLFQL VSDIDAEYFP PREDVILQNE 
421:	APTDLYILVS GAVDFTVYVG EEDQVQGKAV VGDAFGEIGV LCYTPQPFTV RTTELSQILR 
481:	ISKKSLMSAM RAHVEDGRVI MNNLFMKLRG QQSIAIDDPN SEPESLLKEW LVGGSKTGEG 
541:	NASDQGHGHK YLQLHDSENI DMGSTEWRDS RRSGYGETKR VREHTIEIEE GEKPNKEFDG 
601:	KGCSDADLTS FEFHSQEAYP YCRSNIQIKQ HEAAKPKDKR VTIHLKSRDK DLSKLIILPA 
661:	SIEELLRLAG EKFGYSFTKV TNAENAEIDD EDVIRDGDHL YILINENS