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1.C.97.1.1
Pleurotolysin A/B pore-forming toxin. Pleurotolysin A (PlyA; also called ostreolysin A, OlyA) binds first in a sphingomyelin-dependent process; Pleurotolysin B (PlyS) binds to A in the membrane and inserts (Kondos et al., 2011).  The binary cytolytic pore-forming complex forms non-selective ion conducting pores of variable size (Schlumberger et al. 2013) to promote fruiting (Ota et al. 2014). Conformational changes accompanying pore formation have been reported (Lukoyanova et al. 2015).   In these systems, the aegerolysin-like proteins provide the membrane cholesterol/sphingomyelin selectivity and recruit oligomerised pleurotolysin B molecules, to create a membrane-inserted pore complex. The resulting protein structure has been imaged with electron microscopy, and it has a 13-meric rosette-like structure, with a central lumen that is ~4-5 nm in diameter. The opened transmembrane pore is non-selectively permeable for ions and smaller neutral solutes, and is a cause of cytolysis of a colloid-osmotic type (Ota et al. 2014).

Accession Number:Q5W9E8
Protein Name:Pleurotolysin B
Length:523
Molecular Weight:57094.00
Species:Pleurotus ostreatus (Oyster mushroom) [5322]
Substrate small molecules

Cross database links:

Pfam: PF01823   

References (1)

[1] “Cloning, expression, and pore-forming properties of mature and precursor forms of pleurotolysin, a sphingomyelin-specific two-component cytolysin from the edible mushroom Pleurotus ostreatus.”  Sakurai N.et.al.   15245918
Structure:
4OEJ   4OV8   4V2T   4V3A   4V3M   4V3N     

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FASTA formatted sequence
1:	MEAVLSRQAA TAEAIGRFQD SSTSVGLVAG SPSTRIRRQA DNVVLKSTSQ AGDTLNDVIQ 
61:	DPTRRNKLIN DNNLLKGIIM GRDGPVPSSR ELIVRPDTLR AIINNRATIE TTTMEAEFTE 
121:	TLMESNYNSA SVKVSAPFIT ANSEYSESSS FKNTETEKSM YTSSRYLFPQ GRIDFTTPDS 
181:	GFDDVIKLSP QFTSGVQAAL AKATGTEKRE ALQNLFQEYG HVFRTKVHIG GVLSAHTMET 
241:	FSRSENETEV KQDVKAGLEG AVKGWGGGAT AGHGNTQGTI TTSQNRKLNV KYIVNGGDYT 
301:	KIQNTEEWVA STNQSEHWRV IEVTEVTAVA DLLPQPIRGQ VKDLLKPLLG KWVDVEKVPG 
361:	LESLPVSVYR PKGAIPAGWF WLGDTADASK ALLVKPTLPA RSGRNPALTS LHQGSGMTEQ 
421:	PFVDLPQYQY LSTYFGSFAH DTPPGSTLRG LRPDHVLPGR YEMHGDTIST AVYVTRPVDV 
481:	PFPEDECFDL KSLVRVKLPG SGNPPKPRSA LKKSMVLFDS GEK