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3.A.1.23.4
The NikM2 (230 aas; 5 TMSs)/NikN2 (110 aas; 2 TMSs) pair is part or all of a nickel transporter.  The crystal structure of NikM2 is known (PDB 4M5C; 4M58).  It possesses an additional TMS at its N-terminal region not present on other ECF transporter of known structure, resulting in an extracellular N-terminus. The highly conserved N-terminal loop inserts into the center of NikM2 and occludes a region corresponding to the substrate-binding sites of the vitamin-specific S component. Nickel binds to NikM2 by coordination to four nitrogen atoms in Met1, His2 and His67. These nitrogens form a square-planar geometry, similar to that of the metal ion-binding sites in the amino-terminal Cu2+- and Ni2+-binding (ATCUN) motif (Yu et al. 2013).  Constituents other than NikN2 and NikM2 are not known but may be required for activity (T. Eitinger, personal communication).

Accession Number:Q8R9C0
Protein Name:Cobalamin biosynthesis protein CbiM
Length:230
Molecular Weight:24283.00
Species:Thermoanaerobacter tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) [273068]
Number of TMSs:5
Substrate Ni2+

Cross database links:

Structure:
4M58   4M5B   4M5C     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MHIPEGYLSP QTCAVMGAAM VPVLTVAAKK VNKSFDKKDV PAMAIGSAFA FTIMMFNVPI 
61:	PGGTTAHAIG ATLLATTLGP WAASISLTLA LFIQALLFGD GGILALGANS FNMAFIAPFV 
121:	GYGIYRLMLS LKLNKVLSSA IGGYVGINAA ALATAIELGL QPLLFHTANG TPLYFPYGLN 
181:	VAIPAMMFAH LTVAGIVEAV ITGLVVYYLQ KVDEENILHK FSYRLRGDNR