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3.A.2.2.4
The ubiquitous 14 subunit vacuolar H+-ATPase, V1/V0, has been implicated in various human diseases including osteopetrosis, renal tubule acidosis, and cancer (Hinton et al., 2009).  The transmembrane enzyme, Ribonuclease kappa, RNASEK (137 aas; 2 TMSs; UniProt acc# Q6P5S7), closely associates with the V-ATPase and is required for its function; its loss prevents the early events of endocytosis and the replication of multiple pathogenic viruses (Perreira et al. 2015).

Accession Number:Q93050
Protein Name:V-ATPase 116 kDa isoform a1
Length:837
Molecular Weight:96413.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:8
Location1 / Topology2 / Orientation3: Cytoplasmic vesicle membrane1 / Multi-pass membrane protein2
Substrate H+

Cross database links:

Genevestigator: Q93050
RefSeq: NP_001123492.1    NP_001123493.1    NP_005168.2   
Entrez Gene ID: 535   
Pfam: PF01496   
Drugbank: Drugbank Link   
OMIM: 192130  gene
KEGG: hsa:535   

Gene Ontology

GO:0030659 C:cytoplasmic vesicle membrane
GO:0016021 C:integral to membrane
GO:0042470 C:melanosome
GO:0005886 C:plasma membrane
GO:0033177 C:proton-transporting two-sector ATPase compl...
GO:0051117 F:ATPase binding
GO:0015078 F:hydrogen ion transmembrane transporter acti...
GO:0015986 P:ATP synthesis coupled proton transport

References (4)

[1] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[2] “The full-ORF clone resource of the German cDNA consortium.”  Bechtel S.et.al.   17974005
[3] “Proteomic analysis of early melanosomes: identification of novel melanosomal proteins.”  Basrur V.et.al.   12643545
[4] “Proteomic and bioinformatic characterization of the biogenesis and function of melanosomes.”  Chi A.et.al.   17081065

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGELFRSEEM TLAQLFLQSE AAYCCVSELG ELGKVQFRDL NPDVNVFQRK FVNEVRRCEE 
61:	MDRKLRFVEK EIRKANIPIM DTGENPEVPF PRDMIDLEAN FEKIENELKE INTNQEALKR 
121:	NFLELTELKF ILRKTQQFFD EMADPDLLEE SSSLLEPSEM GRGTPLRLGF VAGVINRERI 
181:	PTFERMLWRV CRGNVFLRQA EIENPLEDPV TGDYVHKSVF IIFFQGDQLK NRVKKICEGF 
241:	RASLYPCPET PQERKEMASG VNTRIDDLQM VLNQTEDHRQ RVLQAAAKNI RVWFIKVRKM 
301:	KAIYHTLNLC NIDVTQKCLI AEVWCPVTDL DSIQFALRRG TEHSGSTVPS ILNRMQTNQT 
361:	PPTYNKTNKF TYGFQNIVDA YGIGTYREIN PAPYTIITFP FLFAVMFGDF GHGILMTLFA 
421:	VWMVLRESRI LSQKNENEMF STVFSGRYII LLMGVFSMYT GLIYNDCFSK SLNIFGSSWS 
481:	VRPMFTYNWT EETLRGNPVL QLNPALPGVF GGPYPFGIDP IWNIATNKLT FLNSFKMKMS 
541:	VILGIIHMLF GVSLSLFNHI YFKKPLNIYF GFIPEIIFMT SLFGYLVILI FYKWTAYDAH 
601:	TSENAPSLLI HFINMFLFSY PESGYSMLYS GQKGIQCFLV VVALLCVPWM LLFKPLVLRR 
661:	QYLRRKHLGT LNFGGIRVGN GPTEEDAEII QHDQLSTHSE DADEPSEDEV FDFGDTMVHQ 
721:	AIHTIEYCLG CISNTASYLR LWALSLAHAQ LSEVLWTMVI HIGLSVKSLA GGLVLFFFFT 
781:	AFATLTVAIL LIMEGLSAFL HALRLHWVEF QNKFYSGTGF KFLPFSFEHI REGKFEE