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3.A.2.2.4
The ubiquitous 14 subunit vacuolar H+-ATPase, V1/V0, has been implicated in various human diseases including osteopetrosis, renal tubule acidosis, and cancer (Hinton et al., 2009).  The transmembrane enzyme, Ribonuclease kappa, RNASEK (137 aas; 2 TMSs; UniProt acc# Q6P5S7), closely associates with the V-ATPase and is required for its function; its loss prevents the early events of endocytosis and the replication of multiple pathogenic viruses (Perreira et al. 2015).

Accession Number:Q9UI12
Protein Name:V-ATPase subunit H
Length:483
Molecular Weight:55883.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Endomembrane system1 / Peripheral membrane protein2
Substrate H+

Cross database links:

Genevestigator: Q9UI12
eggNOG: prNOG04128
HEGENOM: HBG387727
RefSeq: NP_057025.2    NP_998784.1    NP_998785.1   
Entrez Gene ID: 51606   
Pfam: PF03224    PF11698   
Drugbank: Drugbank Link   
OMIM: 608861  gene
KEGG: hsa:51606   

Gene Ontology

GO:0005829 C:cytosol
GO:0005886 C:plasma membrane
GO:0000221 C:vacuolar proton-transporting V-type ATPase,...
GO:0030234 F:enzyme regulator activity
GO:0005515 F:protein binding
GO:0046961 F:proton-transporting ATPase activity, rotati...
GO:0015991 P:ATP hydrolysis coupled proton transport
GO:0015986 P:ATP synthesis coupled proton transport
GO:0006897 P:endocytosis
GO:0044419 P:interspecies interaction between organisms
GO:0050690 P:regulation of defense response to virus by ...
GO:0007035 P:vacuolar acidification

References (7)

[1] “Interactions between HIV1 Nef and vacuolar ATPase facilitate the internalization of CD4.”  Lu X.et.al.   9620685
[2] “Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.”  Hu R.-M.et.al.   10931946
[3] “Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics.”  Lai C.-H.et.al.   10810093
[4] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[5] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[6] “Subunit H of the V-ATPase binds to the medium chain of adaptor protein complex 2 and connects Nef to the endocytic machinery.”  Geyer M.et.al.   12032142
[7] “Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.”  Daub H.et.al.   18691976

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTKMDIRGAV DAAVPTNIIA AKAAEVRANK VNWQSYLQGQ MISAEDCEFI QRFEMKRSPE 
61:	EKQEMLQTEG SQCAKTFINL MTHICKEQTV QYILTMVDDM LQENHQRVSI FFDYARCSKN 
121:	TAWPYFLPML NRQDPFTVHM AARIIAKLAA WGKELMEGSD LNYYFNWIKT QLSSQKLRGS 
181:	GVAVETGTVS SSDSSQYVQC VAGCLQLMLR VNEYRFAWVE ADGVNCIMGV LSNKCGFQLQ 
241:	YQMIFSIWLL AFSPQMCEHL RRYNIIPVLS DILQESVKEK VTRIILAAFR NFLEKSTERE 
301:	TRQEYALAMI QCKVLKQLEN LEQQKYDDED ISEDIKFLLE KLGESVQDLS SFDEYSSELK 
361:	SGRLEWSPVH KSEKFWRENA VRLNEKNYEL LKILTKLLEV SDDPQVLAVA AHDVGEYVRH 
421:	YPRGKRVIEQ LGGKQLVMNH MHHEDQQVRY NALLAVQKLM VHNWEYLGKQ LQSEQPQTAA 
481:	ARS