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1.A.1.3.5
Sperm Slo3 high conductance K+ channel, activated by voltage and intracellular alkalinization. In sperm, it gives rise to pH-dependent outwardly rectifying K+ currents. (required for the ensuing acrosome reaction; activated by phosphatidylinositol 4,5-bisphosphate (PIP(2)) (Tang et al., 2010).

Accession Number:O54982
Protein Name:Potassium channel subfamily U member 1
Length:1121
Molecular Weight:126870.00
Species:Mus musculus (Mouse) [10090]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: O54982
eggNOG: roNOG10812
HEGENOM: HBG386987
Entrez Gene ID: 16532   
Pfam: PF03493    PF00520   
KEGG: mmu:16532   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005488 F:binding
GO:0015269 F:calcium-activated potassium channel activity
GO:0003824 F:catalytic activity
GO:0005244 F:voltage-gated ion channel activity

References (6)

[1] “Slo3, a novel pH-sensitive K+ channel from mammalian spermatocytes.”  Schreiber M.et.al.   9452476
[2] “Intracellular Mg(2+) enhances the function of BK-type Ca(2+)-activated K(+) channels.”  Shi J.et.al.   11696614
[3] “Gating and conductance properties of BK channels are modulated by the S9-S10 tail domain of the alpha subunit. A study of mSlo1 and mSlo3 wild-type and chimeric channels.”  Moss B.L.et.al.   11723163
[4] “Ligand-dependent activation of Slo family channels is defined by interchangeable cytosolic domains.”  Xia X.-M.et.al.   15201331
[5] “Slo3 K+ channels: voltage and pH dependence of macroscopic currents.”  Zhang X.et.al.   16940555
[6] “pH-regulated Slo3 K+ channels: properties of unitary currents.”  Zhang X.et.al.   16940554

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSQTLLDSLN QKELTETSCT IEIQAAFILS SLATFFGGLI ILFLFRIALK SSRSWKYVKG 
61:	PRGLLELFSS RRIEANPLRK LYFHGVFRQR IEMLLSAQTV VGQVLVILVF VLSIGSLVIY 
121:	FINSMDPVRR CSSYEDKIVH GDLSFNAFFS FYFGLRFWAA EDKIKFWLEM NSIVDIFTIP 
181:	PTFISYYLKS NWLGLRFLRA LRLLELPKIL QILQVIKTSN SVKLSKLLSI VISTWFTAAG 
241:	FLHLVENSGD PWLNGRNSQT MSYFESIYLV TATMSTVGFG DVVAKTSLGR IFIVFFTLGS 
301:	LILFANYIPE MVELFSTRKK YTKPYEAVKG KKFIVVCGNI TVDSVTAFLR NFLHWKSGEI 
361:	NIEIVFLGET LPCLELETLL KCHTSCTNFV CGTALKFEDL KRVAVENSEA CLILANHFCS 
421:	DLHDEDNSNI MRVLSIKNYY PQTRVIIQIL QSQNKVFLSK IPNWDWSAGD NILCFAELKL 
481:	GFIAQGCLVP GLCTFLTTLF IEQNQKVFPK HPWQKHFLNG LKNKILTQRL SNDFVGMTFP 
541:	QVSRLCFVKL NLMLIAIQHK PFFHSCCTLI LNPSSQVRLN KDTLGFFIAD SSKAVKRAFF 
601:	YCSNCHSDVC NPELIGKCNC KIKSRQQLIA PTIMVMKSSL TDFTTSSHIH ASMSTEIHTC 
661:	FSREQPSLIT ITTNRPTTND TVDDTDMLDS SGMFHWCRAM PLDKVVLKRS EKAKHEFQNH 
721:	IVVCVFGDAQ CTLVGLRNFV MPLRASNYTR QELKDIVFIG SLEYFQREWR FLRNFPKIHI 
781:	MPGSALYMGD LIAVNVEQCS MCVILATPYK ALSSQILVDT EAIMATLNIQ SLRITSPTPG 
841:	SSKSEVKPSS AFDSKERKQR YKQIPILTEL KNPSNIHFIE QMGGLDGMLK GTSLHLSTSF 
901:	STGAVFSDTF LDSLLATSFY NYHVVELLQM LVTGGISSEM EHYLVKEKPY KTTDDYEAIK 
961:	SGRTRCKLGL LSLDQTVLSG INPRKTFGQL FCGSLDNFGI LCVGLYRMID EEEPSQEHKR 
1021:	FVITRPSNEC HLLPSDLVFC AIPFNTTCGK SDSSPSIQAQ NNSTNATTPL AQGSNFFDSH 
1081:	HADESHDLYP VDDTGERWSQ HHHSRVYPLD TLDASDIVQE K