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1.A.1.5.7
The cyclic nucleotide-gated ion (K+, Rb+, Cs+) channel, HLM1 (CNGC4) (mediates the hypersensitive response (HR) of plants in programmed cell death.) Mutants show abnormal cell death and resistance to infection by Pseudomonas syringae (Balagué et al., 2003; Zelman et al., 2012).

Accession Number:Q94AS9
Protein Name:Cyclic nucleotide-gated ion channel 4 aka CNGC4 aka HLM1
Length:694
Molecular Weight:80081.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate K+, Rb+, Cs+

Cross database links:

Genevestigator: Q94AS9
eggNOG: KOG0498
HEGENOM: HBG599469
RefSeq: NP_200236.1    NP_851188.1   
Entrez Gene ID: 835513   
Pfam: PF00027   
KEGG: ath:AT5G54250   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005516 F:calmodulin binding
GO:0030552 F:cAMP binding
GO:0008324 F:cation transmembrane transporter activity
GO:0030553 F:cGMP binding
GO:0005216 F:ion channel activity
GO:0006811 P:ion transport
GO:0009626 P:plant-type hypersensitive response

References (5)

[1] “Characterisation of a novel gene family of putative cyclic nucleotide- and calmodulin-regulated ion channels in Arabidopsis thaliana.”  Koehler C.et.al.   10341447
[2] “Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones.”  Kaneko T.et.al.   9679202
[3] “Empirical analysis of transcriptional activity in the Arabidopsis genome.”  Yamada K.et.al.   14593172
[4] “Phylogenetic relationships within cation transporter families of Arabidopsis.”  Maeser P.et.al.   11500563
[5] “HLM1, an essential signaling component in the hypersensitive response, is a member of the cyclic nucleotide-gated channel ion channel family.”  Balague C.et.al.   12566578

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MATEQEFTRA SRFSRDSSSV GYYSEEDNTE EEDEEEEEME EIEEEEEEEE EEDPRIGLTC 
61:	GGRRNGSSNN NKWMMLGRIL DPRSKWVREW NKVFLLVCAT GLFVDPLFLY TLSVSDTCMC 
121:	LLVDGWLALT VTALRSMTDL LHLWNIWIQF KIARRWPYPG GDSDGDTNKG GGTRGSTRVA 
181:	PPYVKKNGFF FDLFVILPLP QVVLWVVIPS LLKRGSVTLV VSVLLVTFLF QYLPKIYHSI 
241:	RHLRRNATLS GYIFGTVWWG IALNMIAYFV AAHAAGACWY LLGVQRSAKC LKEQCENTIG 
301:	CDLRMLSCKE PVYYGTTVMV LDRARLAWAQ NHQARSVCLD INTNYTYGAY QWTIQLVSSE 
361:	SRLEKILFPI FWGLMTLSTF GNLESTTEWS EVVFNIIVLT SGLLLVTMLI GNIKVFLHAT 
421:	TSKKQAMHLK MRNIEWWMKK RHLPIGFRQR VRNYERQRWA AMRGVDECEM VQNLPEGLRR 
481:	DIKYHLCLDL VRQVPLFQHM DDLVLENICD RVKSLIFTKG ETIQKEGDAV QRMLFVVRGH 
541:	LQSSQLLRDG VKSCCMLGPG NFSGDELLSW CLRRPFVERL PPSSSTLVTL ETTEAFGLDA 
601:	EDVKYVTQHF RYTFVNEKVK RSARYYSPGW RTWAAVAVQL AWRRYKHRLT LTSLSFIRPR 
661:	RPLSRCASLG EDKLRLYAAI LTSPKPNPDD FDDY