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1.A.1.7.3
The 2-pore (4TMS) outward rectifying K+ channel, KCO1 or 2PK1. Possesses two tandem Ca2+-binding EF-hand motifs, and cytosolic free Ca2+ (~300nM) activates (Czempinski et al., 1997).  Reviewed by González et al. 2014.

Accession Number:Q8LBL1
Protein Name:Calcium-activated outward-rectifying potassium channel 1 aka KCO1
Length:363
Molecular Weight:40727.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Vacuole membrane1 / Multi-pass membrane protein2
Substrate K+

Cross database links:

Genevestigator: Q8LBL1
eggNOG: KOG1418
HEGENOM: HBG605035
RefSeq: NP_200374.1    NP_851196.1   
Entrez Gene ID: 835657   
Pfam: PF07885   
BioCyc: MetaCyc:MONOMER-14554   
KEGG: ath:AT5G55630   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005774 C:vacuolar membrane
GO:0005267 F:potassium channel activity
GO:0030007 P:cellular potassium ion homeostasis
GO:0006813 P:potassium ion transport
GO:0051260 P:protein homooligomerization
GO:0010029 P:regulation of seed germination
GO:0010119 P:regulation of stomatal movement

References (6)

[1] “New structure and function in plant K+ channels: KCO1, an outward rectifier with a steep Ca2+ dependency.”  Czempinski K.et.al.   9184204
[2] “Vacuolar membrane localization of the Arabidopsis 'two-pore' K+ channel KCO1.”  Czempinski K.et.al.   12148538
[3] “Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones.”  Sato S.et.al.   9628582
[4] “Phylogenetic relationships within cation transporter families of Arabidopsis.”  Maeser P.et.al.   11500563
[5] “KCO1 is a component of the slow-vacuolar (SV) ion channel.”  Schoenknecht G.et.al.   11821043
[6] “Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks.”  Reiland S.et.al.   19376835

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSSDAARTPL LPTEKIDTMA QDFNLNSRTS SSRKRRLRRS RSAPRGDCMY NDDVKIDEPP 
61:	PHPSKIPMFS DLNPNLRRVI MFLALYLTIG TLCFYLVRDQ ISGHKTSGVV DALYFCIVTM 
121:	TTVGYGDLVP NSSASRLLAC AFVFSGMVLV GHLLSRAADY LVEKQEALLV RAFHLRQSFG 
181:	PTDILKELHT NKLRYKCYAT CLVLVVLFIV GTIFLVMVEK MPVISAFYCV CSTVTTLGYG 
241:	DKSFNSEAGR LFAVFWILTS SICLAQFFLY VAELNTENKQ RALVKWVLTR RITNNDLEAA 
301:	DLDEDGVVGA AEFIVYKLKE MGKIDEKDIS GIMDEFEQLD YDESGTLTTS DIVLAQTTSQ 
361:	IQR