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1.A.10.1.13
Glu2 AMPA receptor (GluR-2; GluA2).  The 3-d structure is known at 3.6 Å resolution.  It shows a 4-fold axis of symmetry in the transmembrane domain, and a 2-fold axis of symmetry overall, although it is a homotetramer (Sobolevsky et al. 2009). A structure showing an agoniar-bound form of the rat GluA2 receptor revealed conformational changes that occur during gating (Yelshanskaya et al. 2014). GluR2 interacts directly with β3 integrin (Pozo et al., 2012).  In general, integrin receptors form macromolelcular complexes with ion channels (Becchetti et al. 2010).  TARPS are required for AMP receptor function and trafficking, but seven other auxiliary subunits have also been identified (Sumioka 2013). For example, AMPA receptors are regulated by S-SCAM through TARPs (Danielson et al. 2012).  The C-terminal domains of various TARPs (TC#8.A.16.2) play direct roles in the regulation of GluRs (Sager et al. 2011).  Whole-genome analyses have linked multiple TARP loci to childhood epilepsy, schizophrenia and bipolar disorders (Kato et al. 2010). Thus, TARPs emerge as vital components of excitatory synapses that participate both in signal transduction and in neuropsychiatric disorders. The architecture of a fully occupied GluR2-TARP complex has been illucidated by cryoEM, showing the homomeric GluA2 AMPA receptor saturated with TARP Υ2 subunits, showing how the TARPs are arranged with four-fold symmetry around the ion channel domain, making extensive interactions with the M1, M2 and M4 TMSs (Zhao et al. 2016).

Accession Number:P42262
Protein Name:Glutamate receptor 2
Length:883
Molecular Weight:98821.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ions

Cross database links:

Genevestigator: P42262
eggNOG: NOG316680
DIP: DIP-42852N
Entrez Gene ID: 2891   
Pfam: PF01094    PF00060    PF10613   
Drugbank: Drugbank Link   
KEGG: hsa:2891   

Gene Ontology

GO:0032281 C:alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex
GO:0030054 C:cell junction
GO:0030666 C:endocytic vesicle membrane
GO:0005789 C:endoplasmic reticulum membrane
GO:0045211 C:postsynaptic membrane
GO:0004971 F:alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity
GO:0005234 F:extracellular-glutamate-gated ion channel activity
GO:0015277 F:kainate selective glutamate receptor activity
GO:0007268 P:synaptic transmission

References (5)

[1] “Primary structure and functional expression of the AMPA/kainate receptor subunit 2 from human brain.”  Sun W.et.al.   8003671
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “RNA editing of the glutamate receptor subunits GluR2 and GluR6 in human brain tissue.”  Paschen W.et.al.   7523595
[4] “Glutamatergic plasticity by synaptic delivery of GluR-B(long)-containing AMPA receptors.”  Kolleker A.et.al.   14687553
[5] “The PDZ domain of PICK1 differentially accepts protein kinase C-alpha and GluR2 as interacting ligands.”  Dev K.K.et.al.   15247289
Structure:
2WJW   2WJX   2XHD   3R7X   3RN8   3RNN   3UA8   3kg2   4u1w   4u1x   [...more]

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MQKIMHISVL LSPVLWGLIF GVSSNSIQIG GLFPRGADQE YSAFRVGMVQ FSTSEFRLTP 
61:	HIDNLEVANS FAVTNAFCSQ FSRGVYAIFG FYDKKSVNTI TSFCGTLHVS FITPSFPTDG 
121:	THPFVIQMRP DLKGALLSLI EYYQWDKFAY LYDSDRGLST LQAVLDSAAE KKWQVTAINV 
181:	GNINNDKKDE MYRSLFQDLE LKKERRVILD CERDKVNDIV DQVITIGKHV KGYHYIIANL 
241:	GFTDGDLLKI QFGGANVSGF QIVDYDDSLV SKFIERWSTL EEKEYPGAHT TTIKYTSALT 
301:	YDAVQVMTEA FRNLRKQRIE ISRRGNAGDC LANPAVPWGQ GVEIERALKQ VQVEGLSGNI 
361:	KFDQNGKRIN YTINIMELKT NGPRKIGYWS EVDKMVVTLT ELPSGNDTSG LENKTVVVTT 
421:	ILESPYVMMK KNHEMLEGNE RYEGYCVDLA AEIAKHCGFK YKLTIVGDGK YGARDADTKI 
481:	WNGMVGELVY GKADIAIAPL TITLVREEVI DFSKPFMSLG ISIMIKKPQK SKPGVFSFLD 
541:	PLAYEIWMCI VFAYIGVSVV LFLVSRFSPY EWHTEEFEDG RETQSSESTN EFGIFNSLWF 
601:	SLGAFMQQGC DISPRSLSGR IVGGVWWFFT LIIISSYTAN LAAFLTVERM VSPIESAEDL 
661:	SKQTEIAYGT LDSGSTKEFF RRSKIAVFDK MWTYMRSAEP SVFVRTTAEG VARVRKSKGK 
721:	YAYLLESTMN EYIEQRKPCD TMKVGGNLDS KGYGIATPKG SSLRNAVNLA VLKLNEQGLL 
781:	DKLKNKWWYD KGECGSGGGD SKEKTSALSL SNVAGVFYIL VGGLGLAMLV ALIEFCYKSR 
841:	AEAKRMKVAK NAQNINPSSS QNSQNFATYK EGYNVYGIES VKI