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1.A.11.1.2
High affinity ammonia/methylammonia uptake carrier, Amt1 or AmtA (Walter et al., 2008)

Accession Number:P54146
Protein Name:Ammonia channel aka Amt1
Length:452
Molecular Weight:47699.00
Species:Corynebacterium glutamicum (Brevibacterium flavum) [1718]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ammonia, Methylammonia

Cross database links:

HEGENOM: HBG677626
RefSeq: NP_600797.1    YP_225867.1   
Entrez Gene ID: 1019551    3343475   
Pfam: PF00909   
BioCyc: CGLU196627:CG1785-MONOMER   
KEGG: cgb:cg1785    cgl:NCgl1521   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008519 F:ammonium transmembrane transporter activity
GO:0015696 P:ammonium transport
GO:0006950 P:response to stress
GO:0055085 P:transmembrane transport

References (3)

[1] “Functional and genetic characterization of the (methyl)ammonium uptake carrier of Corynebacterium glutamicum.”  Siewe R.M.et.al.   8621394
[2] “The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.”  Kalinowski J.et.al.   12948626
[3] “Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032.”  O'Regan M.et.al.   2666264

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDPSDLAWIL AAFALVSLMF PGLSLLYGGM LGGQHVLNTF MMVMSSLGII SLVYIIYGHG 
61:	LVLGNSIGGW GIIGNPLEYF GFRNIMEDDG TGDLMWAGFY ILFAAISLAL VSSGAAGRMR 
121:	FGAWLVFGVL WFTFVYAPLA HWVFAIDDPE SGYVGGWMKN VLEFHDFAGG TAVHMNAGAS 
181:	GLALAIVLGR RHSMAVRPHN LPLILIGAGL IVAGWFGFNG GTAGGANFLA SYVVVTSLIA 
241:	AAGGMMGFML VERVFSGKPT FFGSATGTIA GLVAITPAAD AVSPLGAFAV GALGAVVSFW 
301:	AISWKKGHRV DDSFDVFAVH GMAGIAGALF VMLFGDPLAP AGVSGVFFGG ELSLLWREPL 
361:	AIIVTLTYAF GVTWLIATIL NKFMTLRITS EAEYEGIDRA EHAESAYHLN SNGIGMATRT 
421:	NFGPEIPEET VPDAVQVGVD KQKIADTRKA SK