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1.A.11.1.3
Low affinity (KM > 3mM) ammonia uptake carrier, AmtB (Walter et al., 2008)

Accession Number:Q79VF1
Protein Name:Ammonia permeases aka AmtB
Length:438
Molecular Weight:45406.00
Species:Corynebacterium glutamicum (Brevibacterium flavum) [1718]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ammonia

Cross database links:

HEGENOM: HBG677626
RefSeq: NP_601264.2    YP_226300.1   
Entrez Gene ID: 1020015    3344138   
Pfam: PF00909   
BioCyc: CGLU196627:CG2261-MONOMER   
KEGG: cgb:cg2261    cgl:NCgl1983   

Gene Ontology

GO:0016020 C:membrane
GO:0008519 F:ammonium transmembrane transporter activity
GO:0055085 P:transmembrane transport

References (2)

[1] “The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins.”  Kalinowski J.et.al.   12948626
[2] “Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins.”  Jakoby M.J.et.al.   10227160

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGADQIAAVS GNSAWMLMSA SLVLLMTPAL ALFYGGMSRQ KSVLNMMMMS FGALGVVTVI 
61:	YLLWGWSMSY GTQSIAGIFA NPFEFFGLKD SIVDADGNYI EGAAGYPNII DIGFQLTFAV 
121:	ISTALISGAL AERVKFSTWL IFSGAWVTLV YFPLAHMVWG GGLLGHNVTG FASWLFGSTD 
181:	GEANIAPIDF AGGTVVHISA GTAALVLAFI VGKRKTFGKA IARPHNLPMV MLGAALLWFG 
241:	WFGFNGGSAF AADGLAGLAW VNTTAATAAA MLGWLATEKF RDGHATSLGA ASGVVAGLVA 
301:	ITPAAGALTP VTSLILGAIG GILACLGVGL KYRFGFDDSL DVVGVHLVAG LWGTVGVGLL 
361:	ATDIGWFSGG GMDGLKLFIV QIVIALVAVV FAGVITALIA FALKATVGWR VDDDVEQQGI 
421:	DTHEHAESAY DTTGPEIR