TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.11.2.2
Ammonia-specific uptake carrier, Amt2. For AMT2 from Arabidopsis thaliana NH4+ is the recruited substrate, but the uncharged form NH3 is conducted.  AtAMT2 partially co-localizes with electrogenic AMTs and conducts methylamine with low affinity (Neuhäuser et al., 2009). This may explain the different capacities of AMTs to accumulate ammonium in the plant cell.

Accession Number:Q9M6N7
Protein Name:Ammonium transporter 2 aka Amt2
Length:475
Molecular Weight:50768.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ammonia

Cross database links:

Genevestigator: Q9M6N7
HEGENOM: HBG677626
RefSeq: NP_181363.1   
Entrez Gene ID: 818409   
Pfam: PF00909   
KEGG: ath:AT2G38290   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015398 F:high affinity secondary active ammonium tra...
GO:0015696 P:ammonium transport
GO:0009624 P:response to nematode
GO:0055085 P:transmembrane transport

References (4)

[1] “Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants.”  Sohlenkamp C.et.al.   10675553
[2] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[3] “Whole genome sequence comparisons and 'full-length' cDNA sequences: a combined approach to evaluate and improve Arabidopsis genome annotation.”  Castelli V.et.al.   14993207
[4] “Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane.”  Sohlenkamp C.et.al.   12481062

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MAGAYDPSLP EVPEWLNKGD NAWQLTAATL VGLQSMPGLV ILYASIVKKK WAVNSAFMAL 
61:	YAFAAVLLCW VLLCYKMAFG EELLPFWGKG GPAFDQGYLK GQAKIPNSNV AAPYFPMATL 
121:	VYFQFTFAAI TTILVAGSVL GRMNIKAWMA FVPLWLIFSY TVGAYSIWGG GFLYQWGVID 
181:	YSGGYVIHLS SGVAGFVAAY WVGPRPKADR ERFPPNNVLL MLAGAGLLWM GWSGFNGGAP 
241:	YAANLTSSIA VLNTNLSAAT SLLVWTTLDV IFFGKPSVIG AIQGMVTGLA GVTPGAGLIQ 
301:	TWAAIIIGVV SGTAPWASMM IIHKKSALLQ KVDDTLAVFY THAVAGLLGG IMTGLFAHPD 
361:	LCVLVLPLPA TRGAFYGGNG GKQLLKQLAG AAFIAVWNVV STTIILLAIR VFIPLRMAEE 
421:	ELGIGDDAAH GEEAYALWGD GEKFDATRHV QQFERDQEAA HPSYVHGARG VTIVL