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1.A.11.3.1
Low-affinity ammonia transporter, Mep1 (Has a pair of conserved his/glu residues; Boeckstaens et al., 2008)

Accession Number:P40260
Protein Name:Ammonium transporter MEP1
Length:492
Molecular Weight:54202.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate ammonia

Cross database links:

Genevestigator: P40260
eggNOG: fuNOG04665
HEGENOM: HBG677626
DIP: DIP-5530N
RefSeq: NP_011636.1   
Entrez Gene ID: 853019   
Pfam: PF00909   
KEGG: sce:YGR121C   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008519 F:ammonium transmembrane transporter activity
GO:0005515 F:protein binding
GO:0015696 P:ammonium transport
GO:0019740 P:nitrogen utilization
GO:0055085 P:transmembrane transport

References (6)

[1] “Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae.”  Marini A.-M.et.al.   8062822
[2] “An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes.”  van Dyck L.et.al.   9046098
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.”  Tettelin H.et.al.   9169869
[4] “A family of ammonium transporters in Saccharomyces cerevisiae.”  Marini A.-M.et.al.   9234685
[5] “Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane.”  Soupene E.et.al.   11486013
[6] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MESRTTGPLT TETYDGPTVA FMILGAALVF FMVPGLGFLY SGLARRKSAL ALIWVVLMAT 
61:	LVGILQWYFW GYSLAFSKSA PNNKFIGNLD SFGFRNVYGK KFDEDAYPEL AYATFQMMFS 
121:	CVNLSIIAGA TAERGRLLPH MVFLFILATI GYCPVTYWIW SPGGWAYQWG VLDWAGGGNI 
181:	EILSAVSGFV YSWFLGKRNE KLLINFRPHN VSLVTLGTSI LWFGWLLFNS ASSLSPNLRS 
241:	VYAFMNTCLS AITGGMTWCL LDYRSEKKWS TVGLCSGIIS GLVAATPSSG CITLYGSLIQ 
301:	GIVAGVVCNF ATKLKYYAKV DDAMDILAEH GVAGVIGLIF NALFGADWVI GMDGTTEHEG 
361:	GWVTHNYKQM YKQIAYIAAS IGYTAAVTAI ICFVLGYIPG MRLRISEEAE EAGMDEDQIG 
421:	EFAYDYVEVR RDYYLWGVDE DSQRSDVNHR VNNAHLAAER SSSGTNSSSD GNGEMIQSEK 
481:	ILPIHQEDPA NR