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1.A.11.3.2
High-affinity ammonia transporter and sensor, Mep2 (also an NH4+ sensor) (Javelle et al., 2003a; Rutherford et al., 2008) (has a pair of conserved his/his residues; mutation to his/glu as in Mep1 leads to uncoupling of transport and sensor functions (Boeckstaens et al., 2008))

Accession Number:P41948
Protein Name:Ammonium transporter MEP2
Length:499
Molecular Weight:53401.00
Species:Saccharomyces cerevisiae (Baker's yeast) [4932]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate ammonia

Cross database links:

Genevestigator: P41948
eggNOG: fuNOG04796
HEGENOM: HBG677626
DIP: DIP-4340N
RefSeq: NP_014257.1   
Entrez Gene ID: 855580   
Pfam: PF00909   
KEGG: sce:YNL142W   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008519 F:ammonium transmembrane transporter activity
GO:0015696 P:ammonium transport
GO:0019740 P:nitrogen utilization
GO:0007124 P:pseudohyphal growth
GO:0055085 P:transmembrane transport

References (7)

[1] “A family of ammonium transporters in Saccharomyces cerevisiae.”  Marini A.-M.et.al.   9234685
[2] “A 43.5 kb segment of yeast chromosome XIV, which contains MFA2, MEP2, CAP/SRV2, NAM9, FKB1/FPR1/RBP1, MOM22 and CPT1, predicts an adenosine deaminase gene and 14 new open reading frames.”  Mallet L.et.al.   8619318
[3] “The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.”  Philippsen P.et.al.   9169873
[4] “The MEP2 ammonium permease regulates pseudohyphal differentiation in Saccharomyces cerevisiae.”  Lorenz M.C.et.al.   9482721
[5] “In vivo N-glycosylation of the mep2 high-affinity ammonium transporter of Saccharomyces cerevisiae reveals an extracytosolic N-terminus.”  Marini A.-M.et.al.   11069679
[6] “Evidence that fungal MEP proteins mediate diffusion of the uncharged species NH(3) across the cytoplasmic membrane.”  Soupene E.et.al.   11486013
[7] “A global topology map of the Saccharomyces cerevisiae membrane proteome.”  Kim H.et.al.   16847258
Structure:
5aex     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSYNFTGTPT GEGTGGNSLT TDLNTQFDLA NMGWIGVASA GVWIMVPGIG LLYSGLSRKK 
61:	HALSLLWASM MASAVCIFQW FFWGYSLAFS HNTRGNGFIG TLEFFGFRNV LGAPSSVSSL 
121:	PDILFAVYQG MFAAVTGALM LGGACERARL FPMMVFLFLW MTIVYCPIAC WVWNAEGWLV 
181:	KLGSLDYAGG LCVHLTSGHG GLVYALILGK RNDPVTRKGM PKYKPHSVTS VVLGTVFLWF 
241:	GWMFFNGGSA GNATIRAWYS IMSTNLAAAC GGLTWMVIDY FRCGRKWTTV GLCSGIIAGL 
301:	VGITPAAGFV PIWSAVVIGV VTGAGCNLAV DLKSLLRIDD GLDCYSIHGV GGCIGSVLTG 
361:	IFAADYVNAT AGSYISPIDG GWINHHYKQV GYQLAGICAA LAWTVTVTSI LLLTMNAIPF 
421:	LKLRLSADEE ELGTDAAQIG EFTYEESTAY IPEPIRSKTS AQMPPPHENI DDKIVGNTDA 
481:	EKNSTPSDAS STKNTDHIV