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1.A.16.1.1
Formate uptake/efflux permease, FocA.  It catalyzes bidirectional transport, has a pentameric aquaporin-like (TC# 1.A.8) structure, and may function by a channel-type mechanism (Falke et al., 2009; Wang et al. 2009). The structure at 2.25 Å resolution has been determined (Wang et al., 2009).  The protein is encoded in an operon with pyruvate-formate lyase, PflB.  A pyruvate:formate antiport mechanism has been suggested (Moraes and Reithmeier 2012). The C-terminal 6 aas are required for formate transport, but not for homopentamer formation (Hunger et al. 2017).  The N-terminus of FocA interacts with PflB, and this interaction is essential for optimal formate translocation (Doberenz et al. 2014). In fact, the GREs, TdcE and PflB, interact with the FNT channel protein, probably to control formate translocation by FocA (Falke et al. 2016).

Accession Number:P0AC23
Protein Name:Probable formate transporter 1 FocA aka FOCA_2 aka B0904
Length:285
Molecular Weight:30991.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate formate

Cross database links:

Genevestigator: P0AC23
EchoBASE: EB1238
EcoGene: EG11258
eggNOG: COG2116
HEGENOM: HBG726755
RefSeq: AP_001534.1    NP_415424.1   
Entrez Gene ID: 945513   
Pfam: PF01226   
BioCyc: EcoCyc:FOCA-MONOMER    ECOL168927:B0904-MONOMER   
KEGG: ecj:JW0887    eco:b0904   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005215 F:transporter activity
GO:0006810 P:transport

References (6)

[1] “Novel transcriptional control of the pyruvate formate-lyase gene: upstream regulatory sequences and multiple promoters regulate anaerobic expression.”  Sawers G.et.al.   2651404
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Isolation and characterization of hypophosphite-resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter.”  Suppmann B.et.al.   8022272
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
Structure:
3kcu   3q7k     

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKADNPFDLL LPAAMAKVAE EAGVYKATKH PLKTFYLAIT AGVFISIAFV FYITATTGTG 
61:	TMPFGMAKLV GGICFSLGLI LCVVCGADLF TSTVLIVVAK ASGRITWGQL AKNWLNVYFG 
121:	NLVGALLFVL LMWLSGEYMT ANGQWGLNVL QTADHKVHHT FIEAVCLGIL ANLMVCLAVW 
181:	MSYSGRSLMD KAFIMVLPVA MFVASGFEHS IANMFMIPMG IVIRDFASPE FWTAVGSAPE 
241:	NFSHLTVMNF ITDNLIPVTI GNIIGGGLLV GLTYWVIYLR ENDHH