TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.A.19.1.1
Matrix protein, M2, an acid activated drug-sensitive proton channel.  Transport involves binding to the four His-37s and transfer to water molecules on the inside of the channel (Acharya et al., 2010).  Functional properties and structure are known (Hong and Degrado 2012). The cytoplasmic tail facilitates proton conduction (Liao et al. 2015).  It is a dimer of dimers (Andreas et al. 2015).  The four TMSs flanking the channel lumen alone seem to determine the proton conduction mechanism (Liang et al. 2016). His-37 forms a planar tetrad in the configuration of the bundle accepting and translocating the incoming protons from the N terminal side, exterior of the virus, to the C terminal side, inside the virus (Kalita and Fischer 2017Kalita and Fischer 2017).

Accession Number:P35938
Protein Name:VMT2 aka 7
Length:97
Molecular Weight:11205.00
Species:Influenza A virus (strain A/USSR/90/77) [381516]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Virion membrane1 / Single-pass type III membrane protein2
Substrate H+

Cross database links:

Pfam: PF00599   

Gene Ontology

GO:0020002 C:host cell plasma membrane
GO:0016021 C:integral to membrane
GO:0055036 C:virion membrane
GO:0015078 F:hydrogen ion transmembrane transporter acti...
GO:0005216 F:ion channel activity
GO:0015992 P:proton transport

References (5)

[1] “Primary structure of fragment 7 of the influenza virus A/USSR/90/77(H1N1) RNA.”  Samokhvalov E.I.et.al.   3877509
[2] “Assembly and budding of influenza virus.”  Nayak D.P.et.al.   15567494
[3] “Proton conduction through the M2 protein of the influenza A virus; a quantitative, mechanistic analysis of experimental data.”  Lear J.D.et.al.   12972146
[4] “Computational studies of proton transport through the M2 channel.”  Wu Y.et.al.   12972147
[5] “The closed state of a H+ channel helical bundle combining precise orientational and distance restraints from solid state NMR.”  Nishimura K.et.al.   12403618
Structure:
1NYJ   2H95     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MSLLTEVETP IRNEWGCRCN DSSDPLVVAA SIIGILHLIL WILDRLFFKC IYRLFKHGLK 
61:	RGPSTEGVPE SMREEYRKEQ QNAVDADDSH FVNIELE