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1.A.23.1.1
Minor K+-dependent MscS-type mechanosensitive channel protein, designated KefA, AefA or MscK, (Edwards et al. 2012).

Accession Number:P77338
Protein Name:KefA aka AEFA aka B0465
Length:1120
Molecular Weight:127215.00
Species:Escherichia coli [83333]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate ions

Cross database links:

Genevestigator: P77338
EchoBASE: EB3991
EcoGene: EG14240
eggNOG: COG3264
HEGENOM: HBG296859
DIP: DIP-10070N
RefSeq: AP_001114.1    NP_414998.1   
Entrez Gene ID: 945132   
Pfam: PF00924   
BioCyc: EcoCyc:G6255-MONOMER    ECOL168927:B0465-MONOMER   
KEGG: ecj:JW0454    eco:b0465   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0008381 F:mechanically-gated ion channel activity
GO:0009992 P:cellular water homeostasis
GO:0006813 P:potassium ion transport
GO:0055085 P:transmembrane transport

References (4)

[1] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[2] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[3] “Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity.”  Levina N.et.al.   10202137
[4] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTMFQYYKRS RHFVFSAFIA FVFVLLCQNT AFARASSNGD LPTKADLQAQ LDSLNKQKDL 
61:	SAQDKLVQQD LTDTLATLDK IDRIKEETVQ LRQKVAEAPE KMRQATAALT ALSDVDNDEE 
121:	TRKILSTLSL RQLETRVAQA LDDLQNAQND LASYNSQLVS LQTQPERVQN AMYNASQQLQ 
181:	QIRSRLDGTD VGETALRPSQ KVLMQAQQAL LNAEIDQQRK SLEGNTVLQD TLQKQRDYVT 
241:	ANSARLEHQL QLLQEAVNSK RLTLTEKTAQ EAVSPDEAAR IQANPLVKQE LEINQQLSQR 
301:	LITATENGNQ LMQQNIKVKN WLERALQSER NIKEQIAVLK GSLLLSRILY QQQQTLPSAD 
361:	ELENMTNRIA DLRLEQFEVN QQRDALFQSD AFVNKLEEGH TNEVNSEVHD ALLQVVDMRR 
421:	ELLDQLNKQL GNQLMMAINL QINQQQLMSV SKNLKSILTQ QIFWVNSNRP MDWDWIKAFP 
481:	QSLKDEFKSM KITVNWQKAW PAVFIAFLAG LPLLLIAGLI HWRLGWLKAY QQKLASAVGS 
541:	LRNDSQLNTP KAILIDLIRA LPVCLIILAV GLILLTMQLN ISELLWSFSK KLAIFWLVFG 
601:	LCWKVLEKNG VAVRHFGMPE QQTSHWRRQI VRISLALLPI HFWSVVAELS PLHLMDDVLG 
661:	QAMIFFNLLL IAFLVWPMCR ESWRDKESHT MRLVTITVLS IIPIALMVLT ATGYFYTTLR 
721:	LAGRWIETVY LVIIWNLLYQ TVLRGLSVAA RRIAWRRALA RRQNLVKEGA EGAEPPEEPT 
781:	IALEQVNQQT LRITMLLMFA LFGVMFWAIW SDLITVFSYL DSITLWHYNG TEAGAAVVKN 
841:	VTMGSLLFAI IASMVAWALI RNLPGLLEVL VLSRLNMRQG ASYAITTILN YIIIAVGAMT 
901:	VFGSLGVSWD KLQWLAAALS VGLGFGLQEI FGNFVSGLII LFERPVRIGD TVTIGSFSGT 
961:	VSKIRIRATT ITDFDRKEVI IPNKAFVTER LINWSLTDTT TRLVIRLGVA YGSDLEKVRK 
1021:	VLLKAATEHP RVMHEPMPEV FFTAFGASTL DHELRLYVRE LRDRSRTVDE LNRTIDQLCR 
1081:	ENDINIAFNQ LEVHLHNEKG DEVTEVKRDY KGDDPTPAVG