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1.A.23.4.7
Mechanosensitive ion channel protein 8 (Mechanosensitive channel of small conductance-like 8) (MscS-Like protein 8) is a pollen-specific, membrane tension-gated ion channel required for pollen to survive the hypoosmotic shock of rehydration and for full male fertility. It negatively regulates pollen germination but is required for cellular integrity during germination and tube growth. MSL8 thus senses and responds to changes in membrane tension associated with pollen hydration and germination (Hamilton et al. 2015).

Accession Number:F4IME2
Protein Name:Mechanosensitive ion channel protein 8
Length:908
Molecular Weight:104309.00
Species:Arabidopsis thaliana (Mouse-ear cress) [3702]
Number of TMSs:5
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate ions

Cross database links:

Entrez Gene ID: 816204   
Pfam: PF00924   
KEGG: ath:AT2G17010   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005216 F:ion channel activity

References (2)

[1] “Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.”  Lin X.et.al.   10617197
[2] “Two families of mechanosensitive channel proteins.”  Pivetti C.D.et.al.   12626684

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MDFRNSFKSH SSYKQIRSPG DQSEPSPEHL PILHDHHPDH SGMVVDDQKP DSTRSSLDDG 
61:	RNAPVERDAS YKFWQDNTTG TSTDHTAVRT SDKDPIAISR KGDRLSGSFD FVHGKLPVDE 
121:	SPTKMVAGEP VNRQWRGRNN EEITLDVDQE NDDVSHQTMP TPTSTARTSF DASREMRVSF 
181:	NVRRAGGAFV AGSVPSSSSH SSSSSSATMR TNQDQPQLQE EEVVRCTSNM SFQRKSELIS 
241:	RVKTRSRLQD PPREEETPYS GWRSGQLKSG LLADIDEEDD PLAEEDVPDE YKRGKLDAIT 
301:	LLQWLSLVAI IAALACSLSI QSWKKVRVWN LHLWKWEVFL LVLICGRLVS GWGIRIVVFF 
361:	IERNFLLRKR VLYFVYGVRR AVQNCLWLGL VLLAWHFLFD KKVQRETRSR FLPYVTKILV 
421:	CFLLSTILWL IKTLVVKVLA SSFHVSTYFD RIQEALFNQY VIETLSGPPM IEMSRIEEEE 
481:	ERAQDEIFKM QNAGANLPPD LCAAAFPPGK SGRVMNPKLS PIIPKSTTDN GISMEHLHRM 
541:	NHKNISAWNM KRLMKIVRNV SLTTLDEQML ESTYEDESTR QIRSEKEAKA AARKIFKNVE 
601:	QRGAKYIYLE DLMRFLREDE AMKTMGLFEG APENKRISKS ALKNWLVNAF RERRALALTL 
661:	NDTKTAVNKL HHMINIVTAI VIVVIWLVLL EIASSKVLLF VSSQVVLLAF IFGNTVKTVF 
721:	ESIIFLFIVH PYDVGDRCEI DSVQLVVEEM NILTTVFLRY DNLKIMYPNS LLWQKSINNY 
781:	YRSPDMGDAI EFCVHITTPL EKISVIKQRI SNYIDNKPEY WYPQAKIIVK DLEDLHIVRL 
841:	AIWPCHRINH QDMAERWTRR AVLVEEVIKI LLELDIQHRF YPLDINVRTM PTVVSSRVPP 
901:	GWSQNQPA