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1.A.24.1.2
Connexin 32 (gap junction β1-protein), CX32 (transports adenosine better than CX43 does; Goldberg et al., 2002).  The carboxyl tail regulates gap junction assembly (Katoch et al. 2015).  The modeled channel pore-facing regions of TMSs 1 and 2 were highly sensitive to tryptophan substitution while lipid-facing regions of TMSs 3 and 4 were variably tolerant. Residues facing a putative intracellular water pocket (the IC pocket) were also sensitive.  Interactions important for voltage gating occurred mainly in the mid-region of the channel in TMS 1. TMS 1 of Cx43 was scanned revealing similar but not identical sensitivities (Brennan et al. 2015).

Accession Number:P08033
Protein Name:Connexin 32 aka Cx32
Length:283
Molecular Weight:32004.00
Species:Rattus norvegicus (Rat) [10116]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate small molecules

Cross database links:

Genevestigator: P08033
eggNOG: maNOG10132
RefSeq: NP_058947.1   
Entrez Gene ID: 29584   
Pfam: PF00029    PF10582   
KEGG: rno:29584   

Gene Ontology

GO:0005922 C:connexon complex
GO:0005783 C:endoplasmic reticulum
GO:0016021 C:integral to membrane
GO:0005243 F:gap junction channel activity
GO:0007154 P:cell communication
GO:0051259 P:protein oligomerization
GO:0015868 P:purine ribonucleotide transport

References (7)

[1] “Molecular cloning of cDNA for rat liver gap junction protein.”  Paul D.L.et.al.   3013898
[2] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[3] “Identification of a rat liver cDNA and mRNA coding for the 28 kDa gap junction protein.”  Heynkes R.et.al.   3017758
[4] “The Mr 28,000 gap junction proteins from rat heart and liver are different but related.”  Nicholson B.J.et.al.   2987225
[5] “Structure of a gap junction gene: rat connexin-32.”  Miller T.et.al.   2852976
[6] “Topological analysis of the major protein in isolated intact rat liver gap junctions and gap junction-derived single membrane structures.”  Zimmer D.B.et.al.   3034905
[7] “Topography of connexin32 in rat liver gap junctions. Evidence for an intramolecular disulphide linkage connecting the two extracellular peptide loops.”  Rahman S.et.al.   1667015

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MNWTGLYTLL SGVNRHSTAI GRVWLSVIFI FRIMVLVVAA ESVWGDEKSS FICNTLQPGC 
61:	NSVCYDHFFP ISHVRLWSLQ LILVSTPALL VAMHVAHQQH IEKKMLRLEG HGDPLHLEEV 
121:	KRHKVHISGT LWWTYVISVV FRLLFEAVFM YVFYLLYPGY AMVRLVKCEA FPCPNTVDCF 
181:	VSRPTEKTVF TVFMLAASGI CIILNVAEVV YLIIRACARR AQRRSNPPSR KGSGFGHRLS 
241:	PEYKQNEINK LLSEQDGSLK DILRRSPGTG AGLAEKSDRC SAC