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1.A.43.1.1
The camphor resistance protein, CrcB or FluC (Hu et al. 1996; Sand et al. 2003).  Exports fluoride selectively over chloride by an anion open channel mechanism (Stockbridge et al. 2013).  The active transporter is a dimer of 4 TMS subunits arranged in an antiparallel transmembrane orientation (Stockbridge et al. 2014).  In bacteria lacking Fluc, F- accumulates when the external medium is acidified as a predicted function of the transmembrane pH gradient. This weak acid accumulation effect, which results from the high pKa (3.4) and membrane permeability of HF, is abolished by Fluc channels (Sand et al. 2003).  Exports fluoride selectively over chloride by an anion open channel mechanism (Stockbridge et al. 2013).  The active transporter is a dimer of 4 TMS subunits arranged in an antiparallel transmembrane orientation (Stockbridge et al. 2014).  In bacteria lacking Fluc, F- accumulates when the external medium is acidified as a predicted function of the transmembrane pH gradient. This weak acid accumulation effect, which results from the high pKa (3.4) and membrane permeability of HF, is abolished by Fluc channels (Ji et al. 2014).

Accession Number:P37002
Protein Name:CrcB aka b0624 aka JW0619
Length:127
Molecular Weight:13777.00
Species:Escherichia coli [83333]
Number of TMSs:4
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate F-, Cl-

Cross database links:

Genevestigator: P37002
EchoBASE: EB2123
EcoGene: EG12209
eggNOG: COG0239
HEGENOM: HBG753179
RefSeq: AP_001273.1    NP_415157.1   
Entrez Gene ID: 945798   
Pfam: PF02537   
BioCyc: EcoCyc:EG12209-MONOMER    ECOL168927:B0624-MONOMER   
KEGG: ecj:JW0619    eco:b0624   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane

References (6)

[1] “Overproduction of three genes leads to camphor resistance and chromosome condensation in Escherichia coli.”  Hu K.H.et.al.   8844142
[2] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[3] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[4] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[5] “Phenotypic characterization of overexpression or deletion of the Escherichia coli crcA, cspE and crcB genes.”  Sand O.et.al.   12904550
[6] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996

External Searches:

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MLQLLLAVFI GGGTGSVARW LLSMRFNPLH QAIPLGTLTA NLIGAFIIGI GFAWFSRMTN 
61:	IDPVWKVLIT TGFCGGLTTF STFSAEVVFL LQEGRFGWAL LNVFVNLLGS FAMTALAFWL 
121:	FSASTAH