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1.A.46.1.2
Bestrophin-2 anion channel, BEST2 or VMD2L1 (PNO3-/PCl- = 2.7) (Sun et al., 2002). It also transports bicarbonate (HCO3-) (Qu and Hartzell 2008).  The mouse orthologue is swell-insensitive, but the first 64 aas of Bestrophin 1 of Drosophila melanogaster allowed it to mediate cell swelling in response to hypo-osmotic stress (Stotz and Clapham 2012). BEST2 and BEST4 are expressed in colonic goblet cells (Ito et al. 2013). The structure of bovine BEST2 has been determined, and differences with BEST1 have been noted (Owji et al. 2020). 

Accession Number:Q8NFU1
Protein Name:Bestrophin-2 aka VMD2L1
Length:509
Molecular Weight:57139.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:7
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate nitrate, Cl-

Cross database links:

Genevestigator: Q8NFU1
eggNOG: prNOG14586
HEGENOM: HBG385747
RefSeq: NP_060152.2   
Entrez Gene ID: 54831   
Pfam: PF01062   
OMIM: 607335  gene
KEGG: hsa:54831   

Gene Ontology

GO:0034707 C:chloride channel complex
GO:0005929 C:cilium
GO:0005886 C:plasma membrane
GO:0005254 F:chloride channel activity
GO:0006811 P:ion transport
GO:0051899 P:membrane depolarization
GO:0007608 P:sensory perception of smell

References (5)

[1] “Three novel human VMD2-like genes are members of the evolutionary highly conserved RFP-TM family.”  Stoehr H.et.al.   12032738
[2] “Structure-function analysis of the bestrophin family of anion channels.”  Tsunenari T.et.al.   12907679
[3] “Complete sequencing and characterization of 21,243 full-length human cDNAs.”  Ota T.et.al.   14702039
[4] “The vitelliform macular dystrophy protein defines a new family of chloride channels.”  Sun H.et.al.   11904445
[5] “Bestrophin Cl- channels are highly permeable to HCO3-.”  Qu Z.et.al.   18400985

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MTVTYTARVA NARFGGFSQL LLLWRGSIYK LLWRELLCFL GFYMALSAAY RFVLTEGQKR 
61:	YFEKLVIYCD QYASLIPVSF VLGFYVTLVV NRWWSQYLCM PLPDALMCVV AGTVHGRDDR 
121:	GRLYRRTLMR YAGLSAVLIL RSVSTAVFKR FPTIDHVVEA GFMTREERKK FENLNSSYNK 
181:	YWVPCVWFSN LAAQARREGR IRDNSALKLL LEELNVFRGK CGMLFHYDWI SVPLVYTQVV 
241:	TIALYSYFLA CLIGRQFLDP AQGYKDHDLD LCVPIFTLLQ FFFYAGWLKV AEQLINPFGE 
301:	DDDDFETNFL IDRNFQVSML AVDEMYDDLA VLEKDLYWDA AEARAPYTAA TVFQLRQPSF 
361:	QGSTFDITLA KEDMQFQRLD GLDGPMGEAP GDFLQRLLPA GAGMVAGGPL GRRLSFLLRK 
421:	NSCVSEASTG ASCSCAVVPE GAAPECSCGD PLLDPGLPEP EAPPPAGPEP LTLIPGPVEP 
481:	FSIVTMPGPR GPAPPWLPSP IGEEEENLA