1.A.46.1.6
Bestrophin-1 (Best1) of 689 aas and 4 TMSs in a 2 + 2 arrangement. The x-ray structure has been determined at 2.85 Å resolution with permeant anions and Ca2+ bound (Kane Dickson et al. 2014). The channel is formed from a pentameric assembly of subunits. Ca2+ binds to the
channel's large cytosolic region. A single ion pore, approximately 95 Å
in length, is located along the central axis and contains at least 15
binding sites for anions. A hydrophobic neck within the pore probably
forms the gate. Phenylalanine residues within it may coordinate
permeating anions via anion-π interactions. Conformational changes
observed near the 'Ca2+ clasp' hint at the mechanism of
Ca2+-dependent gating (Kane Dickson et al. 2014).
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| Accession Number: | E1C3A0 |
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| Protein Name: | Uncharacterized protein |
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| Length: | 689 |
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| Molecular Weight: | 78195.00 |
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| Species: | Gallus gallus (Chicken) [9031] |
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| Number of TMSs: | 5 |
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| Substrate |
anion |
|---|
1: MTVTYTNRVA DARLGTFSQL LLQWKGSIYK LLYSEFLIFI SLYFAISLVY RLILSESQRL
61: MFEKLALYCN SYAELIPVSF VLGFYVSLVV SRWWAQYESI PWPDRIMNLV SCNVDGEDEY
121: GRLLRRTLMR YSNLCSVLIL RSVSTAVYKR FPSMEHVVRA GLMTPEEHKK FESLNSPHNK
181: FWIPCVWFSN LAVKARNEGR IRDSVLLQGI LNELNTLRSQ CGRLYGYDWI SIPLVYTQVV
241: TVAVYSFFLA CLIGRQFLDP EKAYPGHELD LFVPVFTFLQ FFFYAGWLKV AEQLINPFGE
301: DDDDFETNWL IDRNLQVSLM AVDEMHQDLP ILEKDLYWNE PDPQPPYTAA TAEYKRPSFL
361: GSTFDISMQK EEMEFQPLEQ IKENEEANHS TPLLGHLGRL LGVQSPSFSR SSSRMNLLRR
421: RGEPTSPFSH YTYQDMGKSG NISHPRKLRE FDAFISTPFY ERPGFYSAPQ TPISSIPMIF
481: PSRRQGRKKP PALSSIAACS NSLKMSDTYK SQSSLGSGAK ETFIWPTERN KGPDSLVVMV
541: EEEKSNSSSK KSPDHEQQGS FKSLKSLKGS HPPWLTLENA ATTTSNCEQS SAFPQPGNIP
601: PSSSTSFCFS FTPVASPVLE RSPIEVSRSG RDTASRSSNA PPTRETRRAE SPSTNDSGIS
661: LAEGDYVGLM EVIMEASESV CEEQMDQCS