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1.A.7.1.3
ATP-gated NaCl-regulated nonselective cation (Na+, K+ and Ca2+) channel, the P2X purinoreceptor 7 (expands to accommodate large molecules such as NAD, N-methyl-D-glucamine and triethyl ammonium) (Li et al., 2005; Lu et al., 2007). Plays a role in changing pain thresholds. A region called ADSEG in all P2X receptors is located in the M2 domain which aligns with TMS 5 in VIC Kchannels (1.A.1). ADSEG from P2X(7)R forms cation-selective channels in artificial lipid bilayers and biological membranes similar to those of the full length protein (de Souza et al., 2011). Regulated by calmodulin (Roger et al., 2008).  P2XRs allow direct permeation of nanometer-sized dyes (Browne et al. 2013).  Macrophage P2X7 receptors are modulated in response to infection with Leishmania amazonensis so that they become more permeable to anions and less permeable to cations (Marques-da-Silva et al. 2011).  Residues involved in pore conductivity and agonist sensitivity have been identified (Jindrichova et al. 2015) as have residues involved in channel activation (Caseley et al. 2016). The channel opening extends from the pre-TMS 2 region through the outer half of the trihelical TMS 2 channel; the gate and the selectivity filter have been identified (Pippel et al. 2017). The purinergic receptors, P2RX4 and P2RX7, when mutated, affect susceptibility to multiple sclerosis (MS) (Sadovnick et al. 2017). P2X7 may serve as a receptor for the regulation of annexin secretion during macrophage polarization (de Torre-Minguela et al. 2016). These receptors can reduce salivary gland inflammation (Khalafalla et al. 2017). The P2X7 receptor forms ion channels dependent on lipids but independently of its cytoplasmic domain (Karasawa et al. 2017).

Accession Number:Q99572
Protein Name:P2X purinoceptor 7 aka ATP receptor aka P2X7 aka Purinergic receptor aka P2Z receptor
Length:595
Molecular Weight:68586.00
Species:Homo sapiens (Human) [9606]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate sodium ions, calcium ions, inorganic cations

Cross database links:

Genevestigator: Q99572
eggNOG: prNOG13847
HEGENOM: HBG713324
RefSeq: NP_002553.2   
Entrez Gene ID: 5027   
Pfam: PF00864   
OMIM: 602566  gene
KEGG: hsa:5027   

Gene Ontology

GO:0032059 C:bleb
GO:0005737 C:cytoplasm
GO:0005887 C:integral to plasma membrane
GO:0005524 F:ATP binding
GO:0004931 F:extracellular ATP-gated cation channel acti...
GO:0001530 F:lipopolysaccharide binding
GO:0042803 F:protein homodimerization activity
GO:0001614 F:purinergic nucleotide receptor activity
GO:0005102 F:receptor binding
GO:0032060 P:bleb assembly
GO:0007166 P:cell surface receptor linked signaling pathway
GO:0045779 P:negative regulation of bone resorption
GO:0043409 P:negative regulation of MAPKKK cascade
GO:0046931 P:pore complex assembly
GO:0030501 P:positive regulation of bone mineralization
GO:0010524 P:positive regulation of calcium ion transpor...
GO:0045919 P:positive regulation of cytolysis
GO:0051495 P:positive regulation of cytoskeleton organiz...
GO:0050718 P:positive regulation of interleukin-1 beta s...
GO:0051709 P:regulation of killing of cells of another o...
GO:0002028 P:regulation of sodium ion transport
GO:0033198 P:response to ATP
GO:0019233 P:sensory perception of pain

References (7)

[1] “The permeabilizing ATP receptor, P2X7. Cloning and expression of a human cDNA.”  Rassendren F.et.al.   9038151
[2] “Gene structure and chromosomal localization of the human P2X7 receptor.”  Buell G.N.et.al.   9826911
[3] “The finished DNA sequence of human chromosome 12.”  Scherer S.E.et.al.   16541075
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Proteomic and functional evidence for a P2X7 receptor signalling complex.”  Kim M.et.al.   11707406
[6] “A Glu-496 to Ala polymorphism leads to loss of function of the human P2X7 receptor.”  Gu B.J.et.al.   11150303
[7] “The consensus coding sequences of human breast and colorectal cancers.”  Sjoeblom T.et.al.   16959974

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FASTA formatted sequence
1:	MPACCSCSDV FQYETNKVTR IQSMNYGTIK WFFHVIIFSY VCFALVSDKL YQRKEPVISS 
61:	VHTKVKGIAE VKEEIVENGV KKLVHSVFDT ADYTFPLQGN SFFVMTNFLK TEGQEQRLCP 
121:	EYPTRRTLCS SDRGCKKGWM DPQSKGIQTG RCVVYEGNQK TCEVSAWCPI EAVEEAPRPA 
181:	LLNSAENFTV LIKNNIDFPG HNYTTRNILP GLNITCTFHK TQNPQCPIFR LGDIFRETGD 
241:	NFSDVAIQGG IMGIEIYWDC NLDRWFHHCR PKYSFRRLDD KTTNVSLYPG YNFRYAKYYK 
301:	ENNVEKRTLI KVFGIRFDIL VFGTGGKFDI IQLVVYIGST LSYFGLAAVF IDFLIDTYSS 
361:	NCCRSHIYPW CKCCQPCVVN EYYYRKKCES IVEPKPTLKY VSFVDESHIR MVNQQLLGRS 
421:	LQDVKGQEVP RPAMDFTDLS RLPLALHDTP PIPGQPEEIQ LLRKEATPRS RDSPVWCQCG 
481:	SCLPSQLPES HRCLEELCCR KKPGACITTS ELFRKLVLSR HVLQFLLLYQ EPLLALDVDS 
541:	TNSRLRHCAY RCYATWRFGS QDMADFANLP SCCRWRIRKE FPKSEGQYSG FKSPY