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1.A.7.2.1
The osmoregulatory intracellular P2X receptor, P2XA gated by ATP (present in the osmoreulatory organelle, the contractile vacuole) (Fountain et al., 2007). One of five P2X receptors in D. discoideum is localized to the contractile vacuole with the ligand binding domain facing the lumen. Plays a role in Ca2+ signaling, but also is Cl- permeable. May function in osmoregulation (Ludlow et al., 2009).

Accession Number:Q86JM7
Protein Name:P2X receptor A
Length:378
Molecular Weight:42186.00
Species: [44689]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Contractile vacuole membrane1
Substrate Ca2+

Cross database links:

eggNOG: NOG41051
HEGENOM: HBG464524
RefSeq: XP_645378.1   
Entrez Gene ID: 8618267   
Pfam: PF00864   
KEGG: ddi:DDB_0238349   

Gene Ontology

GO:0031164 C:contractile vacuolar membrane
GO:0016021 C:integral to membrane
GO:0005524 F:ATP binding
GO:0015276 F:ligand-gated ion channel activity
GO:0004872 F:receptor activity
GO:0009992 P:cellular water homeostasis
GO:0006971 P:hypotonic response
GO:0006811 P:ion transport
GO:0050848 P:regulation of calcium-mediated signaling

References (4)

[1] “An intracellular P2X receptor required for osmoregulation in Dictyostelium discoideum.”  Fountain S.J.et.al.   17625565
[2] “The genome of the social amoeba Dictyostelium discoideum.”  Eichinger L.et.al.   15875012
[3] “Purinergic-mediated Ca2+ influx in Dictyostelium discoideum.”  Ludlow M.J.et.al.   18486207
[4] “Functional characterization of intracellular Dictyostelium discoideum P2X receptors.”  Ludlow M.J.et.al.   19833731

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MGFSFDWDDI FQYSTVKIVR IRDRRLGILH LSFLVGIVAY IVVYSAIIKK GYLFTEVPIG 
61:	SVRTSLKGPN TFASNLTYCS NQQHNGSTYP FTPLECNYWD EQLALFPVGQ DSTFTCTTRV 
121:	RLSKQEANCN FTDPTCKFVD EPGSAKNIYI ADIESFTILI DHTMYASSSG SQFNAVDLHG 
181:	YILNQDGDEV QIDANGTSIG VSGKPDIMTI GQLLSFGGVS LDQASPVDSN VSIRYDGVVL 
241:	FVFITYSNTY TYSTSDFKYV YSVQQIANTI YDVPETIILE SIHSRLLYKR HGIRVIFIQT 
301:	GTIGSFHFQT LLLTLVSGLG LLAVATTVVD QLAIRLLPQR KSYSSLKFQV TESMSNPMKK 
361:	RITTDEGEDV LYTRIEGL