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1.A.79.1.1
The dsRNA transporter, SID-1 (Systematic RNA interference defective-1).  Forms a gated transmembrane channel (Shih and Hunter 2011; Xu et al. 2013).  May function together with or be regulated by Sid-2, a metal-dependent nucleic acid binding protein (Q9GZC9) (McEwan et al. 2012), Sid-3, a tyrosyl protein kinase (Q10925), named Cdc-42-associated kinase, Ack, in mammals (Jose et al. 2012) and Sid-5 (Q19443) which co-localizes with RAB-7 (Q23146Xu et al. 2013).  May function together with or be regulated by Sid-2, a metal-dependent nucleic acid binding protein (Q9GZC9) (McEwan et al. 2012), Sid-3, a tyrosyl protein kinase (Q10925), named Cdc-42-associated kinase, Ack, in mammals (Jose et al. 2012) and Sid-5 (Q19443) which co-localizes with RAB-7 (Q23146) and RLP-1 (Q11117).  Endocytosis may play a role in dsRNA uptake.

Accession Number:Q9GZC8
Protein Name:SID-1
Length:776
Molecular Weight:87931.00
Species:Caenorhabditis elegans [6239]
Number of TMSs:11
Location1 / Topology2 / Orientation3: Membrane1 / Multi-pass membrane protein2
Substrate dsRNA

Cross database links:

eggNOG: meNOG05803 NOG239532
HEGENOM: HBG385298 HOG000018615
RefSeq: NP_504372.2   
Entrez Gene ID: 178900   
KEGG: cel:C04F5.1    cel:C04F5.1   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005215 F:transporter activity
GO:0016246 P:RNA interference
GO:0005887 C:integral to plasma membrane
GO:0051033 F:RNA transmembrane transporter activity
GO:0033227 P:dsRNA transport

References (8)

[1] “Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.”  Winston W.M.et.al.   11834782
[2] “Genome sequence of the nematode C. elegans: a platform for investigating biology.”  The C. elegans sequencing consortiumet.al.   9851916
[3] “Transport of dsRNA into cells by the transmembrane protein SID-1.”  Feinberg E.H.et.al.   12970568
[4] “Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins.”  Kaji H.et.al.   17761667
[5] “Systemic RNAi in C. elegans requires the putative transmembrane protein SID-1.”  Winston W.M.et.al.   11834782
[6] “Genome sequence of the nematode C. elegans: a platform for investigating biology.”  The C. elegans sequencing consortiumet.al.   9851916
[7] “Transport of dsRNA into cells by the transmembrane protein SID-1.”  Feinberg E.H.et.al.   12970568
[8] “Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins.”  Kaji H.et.al.   17761667

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MIRVYLIILM HLVIGLTQNN STTPSPIITS SNSSVLVFEI SSKMKMIEKK LEANTVHVLR 
61:	LELDQSFILD LTKVAAEIVD SSKYSKEDGV ILEVTVSNGR DSFLLKLPTV YPNLKLYTDG 
121:	KLLNPLVEQD FGAHRKRHRI GDPHFHQNLI VTVQSRLNAD IDYRLHVTHL DRAQYDFLKF 
181:	KTGQTTKTLS NQKLTFVKPI GFFLNCSEQN ISQFHVTLYS EDDICANLIT VPANESIYDR 
241:	SVISDKTHNR RVLSFTKRAD IFFTETEISM FKSFRIFVFI APDDSGCSTN TSRKSFNEKK 
301:	KISFEFKKLE NQSYAVPTAL MMIFLTTPCL LFLPIVINII KNSRKLAPSQ SNLISFSPVP 
361:	SEQRDMDLSH DEQQNTSSEL ENNGEIPAAE NQIVEEITAE NQETSVEEGN REIQVKIPLK 
421:	QDSLSLHGQM LQYPVAIILP VLMHTAIEFH KWTTSTMANR DEMCFHNHAC ARPLGELRAW 
481:	NNIITNIGYT LYGAIFIVLS ICRRGRHEYS HVFGTYECTL LDVTIGVFMV LQSIASATYH 
541:	ICPSDVAFQF DTPCIQVICG LLMVRQWFVR HESPSPAYTN ILLVGVVSLN FLISAFSKTS 
601:	YVRFIIAVIH VIVVGSICLA KERSLGSEKL KTRFFIMAFS MGNFAAIVMY LTLSAFHLNQ 
661:	IATYCFIINC IMYLMYYGCM KVLHSERITS KAKLCGALSL LAWAVAGFFF FQDDTDWTRS 
721:	AAASRALNKP CLLLGFFGSH DLWHIFGALA GLFTFIFVSF VDDDLINTRK TSINIF