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1.A.8.1.1
Glycerol facilitator, GlpF. Transports various polyols with decreasing rates as size increases (Heller et al. 1980); also transports arsenite (As(III) and antimonite (Sb(III)) (Meng et al., 2004).

Accession Number:P0AER0
Protein Name:Glycerol uptake facilitator protein GLPF aka B3927
Length:281
Molecular Weight:29780.00
Species:Escherichia coli [83333]
Number of TMSs:6
Location1 / Topology2 / Orientation3: Cell inner membrane1 / Multi-pass membrane protein2
Substrate straight chain polyols, arsenite, glycerol

Cross database links:

Genevestigator: P0AER0
EchoBASE: EB0391
EcoGene: EG10396
eggNOG: COG0580
HEGENOM: HBG705794
DIP: DIP-47976N
RefSeq: AP_003882.1    NP_418362.1   
Entrez Gene ID: 948422   
Pfam: PF00230   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:GLPF-MONOMER    ECOL168927:B3927-MONOMER   
KEGG: ecj:JW3898    eco:b3927   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0015254 F:glycerol channel activity
GO:0046872 F:metal ion binding
GO:0071288 P:cellular response to mercury ion
GO:0015793 P:glycerol transport
GO:0055085 P:transmembrane transport

References (11)

[1] “Nucleotide sequence of the region encompassing the glpKF operon and its upstream region containing a bent DNA sequence of Escherichia coli.”  Muramatsu S.et.al.   2544860
[2] “Structure and regulation of the glpFK operon encoding glycerol diffusion facilitator and glycerol kinase of Escherichia coli K-12.”  Weissenborn D.L.et.al.   1372899
[3] “Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.”  Plunkett G. IIIet.al.   8346018
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli.”  Heller K.B.et.al.   6998951
[7] “Reconstitution and functional comparison of purified GlpF and AqpZ, the glycerol and water channels from Escherichia coli.”  Borgnia M.J.et.al.   11226336
[8] “Global topology analysis of the Escherichia coli inner membrane proteome.”  Daley D.O.et.al.   15919996
[9] “Structure of a glycerol-conducting channel and the basis for its selectivity.”  Fu D.et.al.   11039922
[10] “Fraternal twins: AQP1 and GlpF.”  Unger V.M.et.al.   11101882
[11] “Control of the selectivity of the aquaporin water channel family by global orientational tuning.”  Tajkhorshid E.et.al.   11964478
Structure:
1FX8   1LDA   1LDF   1LDI     

External Searches:

  • Search: DB with
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  • 2° Structure (Network Protein Sequence Analysis)

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Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MSQTSTLKGQ CIAEFLGTGL LIFFGVGCVA ALKVAGASFG QWEISVIWGL GVAMAIYLTA 
61:	GVSGAHLNPA VTIALWLFAC FDKRKVIPFI VSQVAGAFCA AALVYGLYYN LFFDFEQTHH 
121:	IVRGSVESVD LAGTFSTYPN PHINFVQAFA VEMVITAILM GLILALTDDG NGVPRGPLAP 
181:	LLIGLLIAVI GASMGPLTGF AMNPARDFGP KVFAWLAGWG NVAFTGGRDI PYFLVPLFGP 
241:	IVGAIVGAFA YRKLIGRHLP CDICVVEEKE TTTPSEQKAS L