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1.A.9.9.3
X-ray structures of CLIC of 680 aas and 1N-terminal and 4 C-terminal TMSs with a large N-terminal hydrophilic domain (NTD) from a Desulfofustis deltaproteobacterium have been solved. The protein includes a periplasmic NTD fused to the conventional ligand-binding domain (LBD) (Hu et al. 2020). The NTD consists of two jelly-roll domains interacting across each subunit interface. Binding of Ca2+ at the LBD subunit interface is associated with a closed transmembrane pore, with resolved monovalent cations intracellular to the hydrophobic gate. Accordingly, DeCLIC-injected oocytes conducted currents only upon depletion of extracellular Ca2+. DeCLIC crystallized in the absence of Ca2+ with a wide-open pore and remodeled periplasmic domains, including increased contacts between the NTD and classic LBD agonist-binding sites. Functional, structural, and dynamical properties of DeCLIC paralleled those of sTeLIC, a pLGIC from another symbiotic prokaryote. Based on these DeCLIC structures, the previous structure of bacterial ELIC (the first high-resolution structure of a pLGIC) should be reclassified as a "locally closed" conformation. Structures of DeCLIC in multiple conformations illustrate dramatic conformational state transitions and diverse regulatory mechanisms available to ion channels in pLGICs, particularly involving Ca2+ modulation and periplasmic NTDs (Hu et al. 2020).

Accession Number:ESQ11000.1
Protein Name:ESQ11000.1 hypothetical protein N839_03575 [uncultured Desulfofustis sp. PB-SRB1]
Length:643
Molecular Weight:
Species:uncultured Desulfofustis sp. PB-SRB1 [1385624]
Number of TMSs:5
Substrate

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FASTA formatted sequence
1:	MHNLQQLLPT RSLIWIFSFL TSISIWCTVA HAETEGRVQH FTGYIEDGRG IFYSLPDMKQ 
61:	GDIIYASMQN TGGNLDPLVG IMAEEIDPAV SLGQVLEKAL ASENDLISEL TAVADRIFLG 
121:	WDDDGGKGYS ASLEFTIPRD GTYHIFAGST ITNQRLDKFQ PTYTTGSFQL ILGLNAPQVI 
181:	SGEGEPEGEV FASLASLEIK PEAHVQELEI RLDKDTRYLT QHTRNLQPGD TFHALVEPIG 
241:	EAPLPRLRLT DSGGKPLAFG LIDQPGESVE LNYTCDQDIC ELVVHVDGTD GQKDSGEAVY 
301:	RLLVGINAPN LRESGQTPVG SSVFLESDLV TVGLAVDQIV GVDQRSENFS VVGTLKLSWH 
361:	DPKLGFSPDQ CGCTVKSFED ASIRAVAGEI NLPLPSFSFY NQQGNRWSQN QVIFVTPDGR 
421:	ASYFERFTVT LQAPDFDFLA YPFDRQKFSI KVDLAVPTNM FIFNEIERFQ QVVGDQLGEE 
481:	EWVVTSYSQE ITEVPFERGS TNSRFTTTLL VKRNLEYYIL RIFVPLFLII SVSWVIFFLK 
541:	DYGRQLEVAS GNLLVFVAFN FTISGDLPRL GYLTVLDRFM IVSFCLTAIV VLISVCQKRL 
601:	GAVGKQAVAA QIDTWVLVIY PLVYSLYIIW VYLRFFTDHI GW