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1.B.33.2.1
The chloroplast import-associated channel porin, IAP75 or Toc75 that functions with two receptor GTPases, Toc34 and Toc159 (see 3.A.9, the CEPT family).  It contains a polyglycine sequence (residues 91 - 110) that acts as a "rejection signal" at the outer envelope for protein transport into the chloroplast (Endow et al. 2016).

Accession Number:Q43715
Protein Name:Protein TOC75, chloroplastic
Length:809
Molecular Weight:88269.00
Species: [3888]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Plastid1 / Multi-pass membrane protein2
Substrate proteins

Cross database links:

DIP: DIP-904N
Pfam: PF01103   

Gene Ontology

GO:0009707 C:chloroplast outer membrane
GO:0016021 C:integral to membrane
GO:0015450 F:P-P-bond-hydrolysis-driven protein transmem...
GO:0006886 P:intracellular protein transport

References (11)

[1] “Isolation of components of the chloroplast protein import machinery.”  Schnell D.J.et.al.   7973649
[2] “A component of the chloroplastic protein import apparatus is targeted to the outer envelope membrane via a novel pathway.”  Tranel P.J.et.al.   7781598
[3] “Topology studies of the chloroplast protein import channel Toc75.”  Sveshnikova N.et.al.   11030426
[4] “Copper chloride, an inhibitor of protein import into chloroplasts.”  Seedorf M.et.al.   7601278
[5] “A novel, bipartite transit peptide targets OEP75 to the outer membrane of the chloroplastic envelope.”  Tranel P.J.et.al.   8953773
[6] “Stable association of chloroplastic precursors with protein translocation complexes that contain proteins from both envelope membranes and a stromal Hsp100 molecular chaperone.”  Nielsen E.et.al.   9118955
[7] “The chloroplastic protein import machinery contains a Rieske-type iron-sulfur cluster and a mononuclear iron-binding protein.”  Caliebe A.et.al.   9405363
[8] “Reconstitution of a chloroplast protein import channel.”  Hinnah S.C.et.al.   9405364
[9] “Identification of protein transport complexes in the chloroplastic envelope membranes via chemical cross-linking.”  Akita M.et.al.   9060464
[10] “A polyglycine stretch is necessary for proper targeting of the protein translocation channel precursor to the outer envelope membrane of chloroplasts.”  Inoue K.et.al.   12787247
[11] “Import pathways of chloroplast interior proteins and the outer-membrane protein OEP14 converge at Toc75.”  Tu S.-L.et.al.   15258267

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MRTSVIPNRL TPTLTTHPSR RRNDHITTRT SSLKCHLSPS SGDNNDSFNS SLLKTISTTV 
61:	AVSSAAASAF FLTGSLHSPF PNFSGLNAAA GGGAGGGGGG SSSSGGGGGG WFNGDEGSFW 
121:	SRILSPARAI ADEPKSEDWD SHELPADITV LLGRLSGFKK YKISDILFFD RNKKSKVETQ 
181:	DSFLDMVSLK PGGVYTKAQL QKELESLATC GMFEKVDMEG KTNADGSLGL TISFAESMWE 
241:	RADRFRCINV GLMGQSKPVE MDPDMSEKEK IEFFRRQERE YKRRISSARP CLLPTSVHEE 
301:	IKDMLAEQGR VSARLLQKIR DRVQSWYHEE GYACAQVVNF GNLNTREVVC EVVEGDITKL 
361:	SIQYLDKLGN VVEGNTEGPV VQRELPKQLL PGHTFNIEAG KQALRNINSL ALFSNIEVNP 
421:	RPDEMNEGSI IVEIKLKELE QKSAEVSTEW SIVPGRGGRP TLASLQPGGT ITFEHRNLQG 
481:	LNRSLTGSVT TSNFLNPQDD LAFKMEYAHP YLDGVDNPRN RTLRVSCFNS RKLSPVFTGG 
541:	PGVDEVPSIW VDRAGVKANI TENFSRQSKF TYGLVMEEII TRDESNHICS NGQRVLPNGA 
601:	ISADGPPTTL SGTGIDRMAF LQANITRDNT RFVNGTIVGS RNMFQVDQGL GVGSNFPFFN 
661:	RHQLTVTKFL QLMSVEEGAG KSPPPVLVLH GHYGGCVGDL PSYDAFTLGG PYSVRGYNMG 
721:	EIGAARNILE LAAEIRIPIK GTHVYAFAEH GTDLGSSKDV KGNPTVVYRR MGQGSSYGAG 
781:	MKLGLVRAEY AVDHNSGTGA VFFRFGERF