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1.B.33.2.4
TamA (YftM) of 577 aas; has a 16 transmembrane β-stranded β-barrel with 3 PORTRA domains.  The 2.3 Å crystal structure is known revealing that the barrel is closed by a lid-loop (Gruss et al. 2013).  The C-terminal β-strand of the barrel forms an unusual inward kink, which weakens the lateral barrel wall and creates a gate for substrate access to the lipid bilayer.  TamA is an Omp85 homologue that may function in autotransporter biogenesis together with TamB (TC# 1.B.22.1.2) and OMP85 (Selkrig et al. 2012). The TAM complex likely evolved from an original combination of BamA and TamB, with a later gene duplication event of BamA, giving rise to an additional Omp85 sequence that evolved to be TamA in Proteobacteria and TamL in Bacteroidetes/Chlorobi (Heinz et al. 2015). Possibly TamB nucleates folding of the passenger domain while TamA/B-BamA interact to catalyze β-domain membrane insertion and pore enlargement to facmilitate translocation of partially folded autotransporters (M. Babu et al., unpublished hypothesis).

Accession Number:P0ADE4
Protein Name:Translocation and assembly module TamA
Length:577
Molecular Weight:64796.00
Species:Escherichia coli (strain K12) [83333]
Location1 / Topology2 / Orientation3: Cell outer membrane1
Substrate proteins

Cross database links:

Structure:
2LY3   4BZA   4C00   4n74     

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FASTA formatted sequence
1:	MRYIRQLCCV SLLCLSGSAV AANVRLQVEG LSGQLEKNVR AQLSTIESDE VTPDRRFRAR 
61:	VDDAIREGLK ALGYYQPTIE FDLRPPPKKG RQVLIAKVTP GVPVLIGGTD VVLRGGARTD 
121:	KDYLKLLDTR PAIGTVLNQG DYENFKKSLT SIALRKGYFD SEFTKAQLGI ALGLHKAFWD 
181:	IDYNSGERYR FGHVTFEGSQ IRDEYLQNLV PFKEGDEYES KDLAELNRRL SATGWFNSVV 
241:	VAPQFDKARE TKVLPLTGVV SPRTENTIET GVGYSTDVGP RVKATWKKPW MNSYGHSLTT 
301:	STSISAPEQT LDFSYKMPLL KNPLEQYYLV QGGFKRTDLN DTESDSTTLV ASRYWDLSSG 
361:	WQRAINLRWS LDHFTQGEIT NTTMLFYPGV MISRTRSRGG LMPTWGDSQR YSIDYSNTAW 
421:	GSDVDFSVFQ AQNVWIRTLY DRHRFVTRGT LGWIETGDFD KVPPDLRFFA GGDRSIRGYK 
481:	YKSIAPKYAN GDLKGASKLI TGSLEYQYNV TGKWWGAVFV DSGEAVSDIR RSDFKTGTGV 
541:	GVRWESPVGP IKLDFAVPVA DKDEHGLQFY IGLGPEL