TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.B.35.2.1
The N-acetylneuraminic acid-inducible, anion selective porin, NanC (Condemine et al., 2005). A crystal structure (3.3 Å resolution) is available (2WJQ; Wirth et al., 2009). It forms a 28 Å high 12 stranded β barrel like the autotransporter, NalP. The pore is lined by basic residues (conserved in other KdgM family members) allowing diffusion of acidic oligosaccharides (Wirth et al., 2009).  Single channels of NanC at pH 7.0 have: (1) conductance 100 to 800 pS in 100 mM: KCl to 3 M: KCl), (2) anion over cation selectivity, and (3) two forms of voltage-dependent gating (channel closures above 200 mV). Phosphate interferes with channel conductance (Giri et al. 2012).

Accession Number:P69856
Protein Name:Probable N-acetylneuraminic acid outer membrane channel protein NanC (YjhA)
Length:238
Molecular Weight:27888.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1
Substrate anions, oligosaccharides

Cross database links:

Genevestigator: P69856
EchoBASE: EB2290
EcoGene: EG12389
eggNOG: NOG08858
HEGENOM: HBG416127
RefSeq: AP_004803.1    NP_418731.2   
Entrez Gene ID: 946843   
Pfam: PF06178   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:G7921-MONOMER    ECOL168927:B4311-MONOMER   
KEGG: ecj:JW5778    eco:b4311   

Gene Ontology

GO:0045203 C:integral to cell outer membrane
GO:0005886 C:plasma membrane
GO:0046930 C:pore complex
GO:0015478 F:oligosaccharide transporting porin activity
GO:0015136 F:sialic acid transmembrane transporter activity
GO:0006811 P:ion transport
GO:0015772 P:oligosaccharide transport

References (4)

[1] “Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes.”  Burland V.D.et.al.   7610040
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “Function and expression of an N-acetylneuraminic acid-inducible outer membrane channel in Escherichia coli.”  Condemine G.et.al.   15743943
Structure:
2WJQ   2WJR     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MKKAKILSGV LLLCFSSPLI SQAATLDVRG GYRSGSHAYE TRLKVSEGWQ NGWWASMESN 
61:	TWNTIHDNKK ENAALNDVQV EVNYAIKLDD QWTVRPGMLT HFSSNGTRYG PYVKLSWDAT 
121:	KDLNFGIRYR YDWKAYRQQD LSGDMSRDNV HRWDGYVTYH INSDFTFAWQ TTLYSKQNDY 
181:	RYANHKKWAT ENAFVLQYHM TPDITPYIEY DYLDRQGVYN GRDNLSENSY RIGVSFKL