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1.B.55.1.1
The β-barrel porin with a superhelical domain containing tetratricopeptide repeats, PgaA or YcdS; exports (deacetylated) poly β-1,6-N-acetyl glucosamine (PGA), a biofilm adhesin that may also play a role in immune evasion (Itoh et al., 2008; Cerca and Jefferson 2008).

Accession Number:P69434
Protein Name:Biofilm PGA synthesis protein pgaA
Length:807
Molecular Weight:92207.00
Species:Escherichia coli (strain K12) [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Peripheral membrane protein2
Substrate deacetylated PGA

Cross database links:

Genevestigator: P69434
EchoBASE: EB3625
EcoGene: EG13865
eggNOG: NOG06511
HEGENOM: HBG466902
RefSeq: AP_001653.1    NP_415543.1   
Entrez Gene ID: 945596   
BioCyc: EcoCyc:G6531-MONOMER    ECOL168927:B1024-MONOMER   
KEGG: ecj:JW1010    eco:b1024   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0005886 C:plasma membrane
GO:0005488 F:binding
GO:0022891 F:substrate-specific transmembrane transporte...
GO:0042710 P:biofilm formation
GO:0006810 P:transport

References (4)

[1] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[2] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[3] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[4] “The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation.”  Wang X.et.al.   15090514
Structure:
4y25     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MYSSSRKRCP KTKWALKLLT AAFLAASPAA KSAVNNAYDA LIIEARKGNT QPALSWFALK 
61:	SALSNNQIAD WLQIALWAGQ DKQVITVYNR YRHQQLPARG YAAVAVAYRN LQQWQNSLTL 
121:	WQKALSLEPQ NKDYQRGQIL TLADAGHYDT ALVKLKQLNS GAPDKANLLA EAYIYKLAGR 
181:	HQDELRAMTE SLPENASTQQ YPTEYVQALR NNQLAAAIDD ANLTPDIRAD IHAELVRLSF 
241:	MPTRSESERY AIADRALAQY AALEILWHDN PDRTAQYQRI QVDHLGALLT RDRYKDVISH 
301:	YQRLKKTGQI IPPWGQYWVA SAYLKDHQPK KAQSIMTELF YHKETIAPDL SDEELADLFY 
361:	SHLESENYPG ALTVTQHTIN TSPPFLRLMG TPTSIPNDTW LQGHSFLSTV AKYSNDLPQA 
421:	EMTARELAYN APGNQGLRID YASVLQARGW PRAAENELKK AEVIEPRNIN LEVEQAWTAL 
481:	TLQEWQQAAV LTHDVVEREP QDPGVVRLKR AVDVHNLAEL RIAGSTGIDA EGPDSGKHDV 
541:	DLTTIVYSPP LKDNWRGFAG FGYADGQFSE GKGIVRDWLA GVEWRSRNIW LEAEYAERVF 
601:	NHEHKPGARL SGWYDFNDNW RIGSQLERLS HRVPLRAMKN GVTGNSAQAY VRWYQNERRK 
661:	YGVSWAFTDF SDSNQRHEVS LEGQERIWSS PYLIVDFLPS LYYEQNTEHD TPYYNPIKTF 
721:	DIVPAFEASH LLWRSYENSW EQIFSAGVGA SWQKHYGTDV VTQLGYGQRI SWNDVIDAGA 
781:	TLRWEKRPYD GDREHNLYVE FDMTFRF