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1.B.6.1.1
Weakly anion-selective OmpA porin.  Can exist in two distinct conductance states (Arora et al. 2000).  May function in the transport of phenylpropanoids (resveratrol, naringenin and rutin) (Zhou et al. 2014).

Accession Number:P0A910
Protein Name:Outer membrane protein A OmpA aka TOLG aka TUT aka CON aka B0957
Length:346
Molecular Weight:37201.00
Species:Escherichia coli [83333]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate anions, resveratrol, Naringenin, Rutin

Cross database links:

Genevestigator: P0A910
EchoBASE: EB0663
EcoGene: EG10669
eggNOG: COG2885
HEGENOM: HBG741096
DIP: DIP-31879N
RefSeq: AP_001587.1    NP_415477.1   
Entrez Gene ID: 945571   
Pfam: PF00691   
Drugbank: Drugbank Link   
BioCyc: EcoCyc:EG10669-MONOMER    ECOL168927:B0957-MONOMER   
KEGG: ecj:JW0940    eco:b0957   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0005886 C:plasma membrane
GO:0046930 C:pore complex
GO:0015288 F:porin activity
GO:0005515 F:protein binding
GO:0005198 F:structural molecule activity
GO:0000746 P:conjugation
GO:0009597 P:detection of virus
GO:0046718 P:entry of virus into host cell
GO:0006811 P:ion transport

References (19)

[1] “Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12.”  Beck E.et.al.   6253901
[2] “Gene structure of the OmpA protein, a major surface protein of Escherichia coli required for cell-cell interaction.”  Movva N.R.et.al.   6260961
[3] “A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.”  Oshima T.et.al.   8905232
[4] “The complete genome sequence of Escherichia coli K-12.”  Blattner F.R.et.al.   9278503
[5] “Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.”  Hayashi K.et.al.   16738553
[6] “Primary structure of major outer membrane protein II (ompA protein) of Escherichia coli K-12.”  Chen R.et.al.   7001461
[7] “Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.”  Link A.J.et.al.   9298646
[8] “Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis.”  Molloy M.P.et.al.   9629924
[9] “Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: analysis of mutant genes expressing altered proteins.”  Morona R.et.al.   6086577
[10] “Bacteriophage receptor area of outer membrane protein OmpA of Escherichia coli K-12.”  Morona R.et.al.   3902787
[11] “Pore-forming activity of OmpA protein of Escherichia coli.”  Sugawara E.et.al.   1370823
[12] “Evidence for a loop-like insertion mechanism of pro-Omp A into the inner membrane of Escherichia coli.”  Kuhn A.et.al.   7813480
[13] “Prediction of transmembrane beta-strands from hydrophobic characteristics of proteins.”  Gromiha M.M.et.al.   8106193
[14] “Structural and functional roles of the surface-exposed loops of the beta-barrel membrane protein OmpA from Escherichia coli.”  Koebnik R.et.al.   10368142
[15] “Protein complexes of the Escherichia coli cell envelope.”  Stenberg F.et.al.   16079137
[16] “Structure of the outer membrane protein A transmembrane domain.”  Pautsch A.et.al.   9808047
[17] “High-resolution structure of the OmpA membrane domain.”  Pautsch A.et.al.   10764596
[18] “Structure of outer membrane protein A transmembrane domain by NMR spectroscopy.”  Arora A.et.al.   11276254
[19] “Proteomics on full-length membrane proteins using mass spectrometry.”  le Coutre J.et.al.   10757971
Structure:
1BXW   1G90   1QJP   2GE4   2JMM   3NB3     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKKTAIAIAV ALAGFATVAQ AAPKDNTWYT GAKLGWSQYH DTGFINNNGP THENQLGAGA 
61:	FGGYQVNPYV GFEMGYDWLG RMPYKGSVEN GAYKAQGVQL TAKLGYPITD DLDIYTRLGG 
121:	MVWRADTKSN VYGKNHDTGV SPVFAGGVEY AITPEIATRL EYQWTNNIGD AHTIGTRPDN 
181:	GMLSLGVSYR FGQGEAAPVV APAPAPAPEV QTKHFTLKSD VLFNFNKATL KPEGQAALDQ 
241:	LYSQLSNLDP KDGSVVVLGY TDRIGSDAYN QGLSERRAQS VVDYLISKGI PADKISARGM 
301:	GESNPVTGNT CDNVKQRAAL IDCLAPDRRV EIEVKGIKDV VTQPQA