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1.C.1.2.2
Colicin E1. Ho et al. (2011) suggested a membrane topological model with a circular arrangement of helices 1-7 in a clockwise direction from the extracellular side and membrane interfacial association of helices 1, 6, 7, and 10 around the central transmembrane hairpin formed by helices 8 and 9.  ColE1 induces lipid flipping, consistent with the toroidal (proteolipidic) pore model of channel formation (Sobko et al. 2010).  The mechanism of channel integration involving the transition of the soluble to membrane-bound form has been presented (Lugo et al. 2016).

Accession Number:P02978
Protein Name:CEA1 aka CEA
Length:522
Molecular Weight:57280.00
Species:Escherichia coli [562]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell membrane1 / Multi-pass membrane protein2
Substrate ions

Cross database links:

Pfam: PF01024   

Gene Ontology

GO:0016021 C:integral to membrane
GO:0005886 C:plasma membrane
GO:0005102 F:receptor binding
GO:0019835 P:cytolysis
GO:0050829 P:defense response to Gram-negative bacterium

References (5)

[1] “Nucleotide sequence of the structural gene for colicin E1 and predicted structure of the protein.”  Yamada M.et.al.   6953432
[2] “Nucleotide polymorphism in colicin E1 and Ia plasmids from natural isolates of Escherichia coli.”  Riley M.A.et.al.   7972047
[3] “The nucleotide sequence surrounding the promoter region of colicin E1 gene.”  Ebina Y.et.al.   6271636
[4] “Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity.”  Oka A.et.al.   384144
[5] “Solution NMR studies of colicin E1 C-terminal thermolytic peptide. Structural comparison with colicin A and the effects of pH changes.”  Wormald M.R.et.al.   2199197
Structure:
2I88     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	METAVAYYKD GVPYDDKGQV IITLLNGTPD GSGSGGGGGK GGSKSESSAA IHATAKWSTA 
61:	QLKKTQAEQA ARAKAAAEAQ AKAKANRDAL TQRLKDIVNE ALRHNASRTP SATELAHANN 
121:	AAMQAEDERL RLAKAEEKAR KEAEAAEKAF QEAEQRRKEI EREKAETERQ LKLAEAEEKR 
181:	LAALSEEAKA VEIAQKKLSA AQSEVVKMDG EIKTLNSRLS SSIHARDAEM KTLAGKRNEL 
241:	AQASAKYKEL DELVKKLSPR ANDPLQNRPF FEATRRRVGA GKIREEKQKQ VTASETRINR 
301:	INADITQIQK AISQVSNNRN AGIARVHEAE ENLKKAQNNL LNSQIKDAVD ATVSFYQTLT 
361:	EKYGEKYSKM AQELADKSKG KKIGNVNEAL AAFEKYKDVL NKKFSKADRD AIFNALASVK 
421:	YDDWAKHLDQ FAKYLKITGH VSFGYDVVSD ILKIKDTGDW KPLFLTLEKK AADAGVSYVV 
481:	ALLFSLLAGT TLGIWGIAIV TGILCSYIDK NKLNTINEVL GI