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1.C.11.1.3
Haemolysin A, HlyA (α-haemolysin) (Wiles and Mulvey 2013). The channel-forming domain may contain β-strands, possibly in addition to alpha-helical structures (Benz et al. 2014).  Although homologous, HlyA and CyaA (1.C.11.1.4) exhibit different modes of permeabilization (Fiser and Konopásek 2009).

Accession Number:P09983
Protein Name:HLY1 aka HlyA
Length:1023
Molecular Weight:109867.00
Species:Escherichia coli [562]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Secreted1 / Multi-pass membrane protein2
Substrate small molecules

Cross database links:

DIP: DIP-16932N
Pfam: PF00353    PF02382    PF08339   

Gene Ontology

GO:0020002 C:host cell plasma membrane
GO:0016021 C:integral to membrane
GO:0005509 F:calcium ion binding
GO:0019835 P:cytolysis
GO:0019836 P:hemolysis by symbiont of host erythrocytes
GO:0009405 P:pathogenesis

References (3)

[1] “Nucleotide sequence of an Escherichia coli chromosomal hemolysin.”  Felmlee T.et.al.   3891743
[2] “Characterisation of HlyC and mechanism of activation and secretion of haemolysin from E. coli 2001.”  Nicaud J.-M.et.al.   3894051
[3] “Fatty acylation of two internal lysine residues required for the toxic activity of Escherichia coli hemolysin.”  Stanley P.et.al.   7801126

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MPTITAAQIK STLQSAKQSA ANKLHSAGQS TKDALKKAAE QTRNAGNRLI LLIPKDYKGQ 
61:	GSSLNDLVRT ADELGIEVQY DEKNGTAITK QVFGTAEKLI GLTERGVTIF APQLDKLLQK 
121:	YQKAGNKLGG SAENIGDNLG KAGSVLSTFQ NFLGTALSSM KIDELIKKQK SGGNVSSSEL 
181:	AKASIELINQ LVDTAASLNN VNSFSQQLNK LGSVLSNTKH LNGVGNKLQN LPNLDNIGAG 
241:	LDTVSGILSA ISASFILSNA DADTGTKAAA GVELTTKVLG NVGKGISQYI IAQRAAQGLS 
301:	TSAAAAGLIA SVVTLAISPL SFLSIADKFK RANKIEEYSQ RFKKLGYDGD SLLAAFHKET 
361:	GAIDASLTRI STVLASVSSG ISAAATTSLV GAPVSALVGA VTGIISGILE ASKQAMFEHV 
421:	ASKMADVIAE WEKKHGKNYF ENGYDARHAA FLEDNFKILS QYNKEYSVER SVLITQQHWD 
481:	TLIGELAGVT RNGDKTLSGK SYIDYYEEGK RLEKKPDEFQ KQVFDPLKGN IDLSDSKSST 
541:	LLKFVTPLLT PGEEIRERRQ SGKYEYITEL LVKGVDKWTV KGVQDKGSVY DYSNLIQHAS 
601:	VGNNQYREIR IESHLGDGDD KVFLSAGSAN IYAGKGHDVV YYDKTDTGYL TIDGTKATEA 
661:	GNYTVTRVLG GDVKVLQEVV KEQEVSVGKR TEKTQYRSYE FTHINGKNLT ETDNLYSVEE 
721:	LIGTTRADKF FGSKFADIFH GADGDDHIEG NDGNDRLYGD KGNDTLSGGN GDDQLYGGDG 
781:	NDKLIGGAGN NYLNGGDGDD ELQVQGNSLA KNVLSGGKGN DKLYGSEGAD LLDGGEGNDL 
841:	LKGGYGNDIY RYLSGYGHHI IDDDGGKDDK LSLADIDFRD VAFRREGNDL IMYKAEGNVL 
901:	SIGHKNGITF KNWFEKESGD ISNHQIEQIF DKDGRVITPD SLKKALEYQQ SNNKASYVYG 
961:	NDALAYGSQG NLNPLINEIS KIISAAGNFD VKEERAAASL LQLSGNASDF SYGRNSITLT 
1021:	ASA