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1.C.38.1.5
Echotoxin-2 precursor, Echt-2 hemolysin (276 aas). Pore-forming protein; forms cation-selective hydrophilic pores of around 1 nm and causes cardiac stimulation and hemolysis. Pore formation is a multi-step process that involves recognition of membrane sphingomyelin using aromatic rich regions and adjacent phosphocholine binding sites for firm binding to the membrane accompanied by the transfer of the N-terminal region to the lipid-water interface and finally pore formation after oligomerization of several monomers (Kawashima et al., 2003; Shiomi et al., 2002).

Accession Number:Q76CA2
Protein Name:Echotoxin-2 aka Echt-2
Length:276
Molecular Weight:29872.00
Species:Cymatium echo (Giant triton) (Monoplex echo) [230564]
Location1 / Topology2 / Orientation3: Secreted1
Substrate cations

Cross database links:

Gene Ontology

GO:0005576 C:extracellular region
GO:0046930 C:pore complex
GO:0015267 F:channel activity
GO:0006812 P:cation transport
GO:0019835 P:cytolysis
GO:0019836 P:hemolysis by symbiont of host erythrocytes
GO:0009405 P:pathogenesis
GO:0046931 P:pore complex assembly

References (2)

[1] “Primary structure of echotoxin 2, an actinoporin-like hemolytic toxin from the salivary gland of the marine gastropod Monoplex echo.”  Kawashima Y.et.al.   14529730
[2] “Properties of proteinaceous toxins in the salivary gland of the marine gastropod (Monoplex echo).”  Shiomi K.et.al.   11821129

External Searches:

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  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKRNILALVV VVALISQSRP AESAGGTIIA TLSKIPLSTL ASALNTALET GASVASAAAA 
61:	ATSSDYSVTC VIEVENWTKH LMKYPVVQIA NSGGLLTVAK NVLPAEIQSF AMRKAWGANG 
121:	VYGTVSWVLG QTNRRVVIMW SAPYNFDFYS NWLAVGMSRP GLAVPSSRST WFDLMYYGNS 
181:	NADISFCTRR ILPTVSTQSI SRTLSGKSEG SMNNIHKARV RATVKPIKTM DLASSILTKL 
241:	EALAGANGKR AIQQELARRA EEEKQRKRKA LDEMLK