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1.C.66.1.1
Puroindoline-a

Accession Number:P33432
Protein Name:Puroindoline-A
Length:148
Molecular Weight:16387.00
Species:Triticum aestivum (Wheat) [4565]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Membrane1
Substrate small molecules

Cross database links:

Pfam: PF00234   

Gene Ontology

GO:0005615 C:extracellular space
GO:0016020 C:membrane
GO:0042742 P:defense response to bacterium
GO:0009405 P:pathogenesis

References (5)

[1] “Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression.”  Gautier M.-F.et.al.   7516201
[2] “Complete amino acid sequence of puroindoline, a new basic and cystine-rich protein with a unique tryptophan-rich domain, isolated from wheat endosperm by Triton X-114 phase partitioning.”  Blochet J.-E.et.al.   8365477
[3] “Puroindolines form ion channels in biological membranes.”  Charnet P.et.al.   12668449
[4] “Two plant puroindolines colocalize in wheat seed and in vitro synergistically fight against pathogens.”  Capparelli R.et.al.   16240178
[5] “Characterization of wheat puroindoline proteins.”  Day L.et.al.   17076702

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MKALFLIGLL ALVASTAFAQ YSEVVGSYDV AGGGGAQQCP VETKLNSCRN YLLDRCSTMK 
61:	DFPVTWRWWK WWKGGCQELL GECCSRLGQM PPQCRCNIIQ GSIQGDLGGI FGFQRDRASK 
121:	VIQEAKNLPP RCNQGPPCNI PGTIGYYW