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1.C.9.1.1
Vacuolating cytotoxin precursor, VacA of 1287 aas that forms a hexameric pore in the membranes of target cells after processing (Pyburn et al. 2016).  The 88 kDa secreted VacA protein, composed of an N-terminal p33 domain and a C-terminal p55 domain, assembles first into water-soluble oligomers before inserting into membranes.  Details for the insertion process are known (Pyburn et al. 2016).  The biology of VacAs has been reviewed (Foegeding et al. 2016).

Accession Number:P55981
Protein Name:VacA aka HP0887
Length:1290
Molecular Weight:139312.00
Species:Helicobacter pylori (Campylobacter pylori) [210]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate small molecules

Cross database links:

HEGENOM: HBG501852
DIP: DIP-3244N
RefSeq: NP_207680.1   
Entrez Gene ID: 899415   
Pfam: PF03797    PF02691   
KEGG: hpy:HP0887   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane
GO:0009405 P:pathogenesis

References (1)

[1] “The complete genome sequence of the gastric pathogen Helicobacter pylori.”  Tomb J.-F.et.al.   9252185

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEIQQTHRKI NRPLVSLALV GALVSITPQQ SHAAFFTTVI IPAIVGGIAT GAAVGTVSGL 
61:	LGWGLKQAEE ANKTPDKPDK VWRIQAGKGF NEFPNKEYDL YRSLLSSKID GGWDWGNAAT 
121:	HYWVKGGQWN KLEVDMKDAV GTYNLSGLRN FTGGDLDVNM QKATLRLGQF NGNSFTSYKD 
181:	SADRTTRVDF NAKNILIDNF LEINNRVGSG AGRKASSTVL TLQASEGITS SKNAEISLYD 
241:	GATLNLASNS VKLMGNVWMG RLQYVGAYLA PSYSTINTSK VTGEVNFNHL TVGDHNAAQA 
301:	GIIASNKTHI GTLDLWQSAG LNIIAPPEGG YKDKPKDKPS NTTQNNANNN QQNSAQNNSN 
361:	TQVINPPNSA QKTEIQPTQV IDGPFAGGKD TVVNIDRINT NADGTIKVGG YKASLTTNAA 
421:	HLHIGKGGIN LSNQASGRTL LVENLTGNIT VDGPLRVNNQ VGGYALAGSS ANFEFKAGTD 
481:	TKNGTATFNN DISLGRFVNL KVDAHTANFK GIDTGNGGFN TLDFSGVTGK VNINKLITAS 
541:	TNVAVKNFNI NELVVKTNGV SVGEYTHFSE DIGSQSRINT VRLETGTRSI FSGGVKFKSG 
601:	EKLVIDEFYY SPWNYFDARN IKNVEITRKF ASSTPENPWG TSKLMFNNLT LGQNAVMDYS 
661:	QFSNLTIQGD FINNQGTINY LVRGGQVATL NVGNAAAMFF SNNVDSATGF YQPLMKINSA 
721:	QDLIKNKEHV LLKAKIIGYG NVSLGTNSIS NVNLIEQFKE RLALYNNNNR MDICVVRNTD 
781:	DIKACGTAIG NQSMVNNPDN YKYLIGKAWK NIGISKTANG SKISVYYLGN STPTEKGGNT 
841:	TNLPTNTTSN VRSANNALAQ NAPFAQPSAT PNLVAINQHD FGTIESVFEL ANRSKDIDTL 
901:	YANSGAQGRD LLQTLLIDSH DAGYARQMID NTSTGEITKQ LNAATTTLNN IASLEHKTSS 
961:	LQTLSLSNAM ILNSRLVNLS RRHTNNIDSF AQRLQALKDQ KFASLESAAE VLYQFAPKYE 
1021:	KPTNVWANAI GGTSLNNGGN ASLYGTSAGV DAYLNGEVEA IVGGFGSYGY SSFNNQANSL 
1081:	NSGANNTNFG VYSRIFANQH EFDFEAQGAL GSDQSSLNFK SALLRDLNQS YNYLAYSAAT 
1141:	RASYGYDFAF FRNALVLKPS VGVSYNHLGS TNFKSNSNQV ALKNGSSSQH LFNASANVEA 
1201:	RYYYGDTSYF YMNAGVLQEF ANFGSSNAVS LNTFKVNAAH NPLSTHARVM MGGELKLAKE 
1261:	VFLNLGFVYL HNLISNIGHF ASNLGMRYSF