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1.C.9.1.1
Vacuolating cytotoxin precursor, VacA of 1287 aas that forms a hexameric pore in the membranes of target cells after processing (Pyburn et al. 2016).  The 88 kDa secreted VacA protein, composed of an N-terminal p33 domain and a C-terminal p55 domain, assembles first into water-soluble oligomers before inserting into membranes.  Details for the insertion process are known (Pyburn et al. 2016).  The biology of VacAs has been reviewed (Foegeding et al. 2016). VacA preferentially associates with lipid rafts, and the affinity of VacA for rafts is independent of its capacity to oligomerize or form membrane channels (Raghunathan et al. 2018).

Accession Number:P55981
Protein Name:VacA aka HP0887
Length:1290
Molecular Weight:139312.00
Species:Helicobacter pylori (Campylobacter pylori) [210]
Number of TMSs:1
Location1 / Topology2 / Orientation3: Cell outer membrane1 / Multi-pass membrane protein2
Substrate small molecules

Cross database links:

HEGENOM: HBG501852
DIP: DIP-3244N
RefSeq: NP_207680.1   
Entrez Gene ID: 899415   
Pfam: PF03797    PF02691   
KEGG: hpy:HP0887   

Gene Ontology

GO:0009279 C:cell outer membrane
GO:0009986 C:cell surface
GO:0005576 C:extracellular region
GO:0016021 C:integral to membrane
GO:0042597 C:periplasmic space
GO:0005886 C:plasma membrane
GO:0009405 P:pathogenesis

References (1)

[1] “The complete genome sequence of the gastric pathogen Helicobacter pylori.”  Tomb J.-F.et.al.   9252185

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MEIQQTHRKI NRPLVSLALV GALVSITPQQ SHAAFFTTVI IPAIVGGIAT GAAVGTVSGL 
61:	LGWGLKQAEE ANKTPDKPDK VWRIQAGKGF NEFPNKEYDL YRSLLSSKID GGWDWGNAAT 
121:	HYWVKGGQWN KLEVDMKDAV GTYNLSGLRN FTGGDLDVNM QKATLRLGQF NGNSFTSYKD 
181:	SADRTTRVDF NAKNILIDNF LEINNRVGSG AGRKASSTVL TLQASEGITS SKNAEISLYD 
241:	GATLNLASNS VKLMGNVWMG RLQYVGAYLA PSYSTINTSK VTGEVNFNHL TVGDHNAAQA 
301:	GIIASNKTHI GTLDLWQSAG LNIIAPPEGG YKDKPKDKPS NTTQNNANNN QQNSAQNNSN 
361:	TQVINPPNSA QKTEIQPTQV IDGPFAGGKD TVVNIDRINT NADGTIKVGG YKASLTTNAA 
421:	HLHIGKGGIN LSNQASGRTL LVENLTGNIT VDGPLRVNNQ VGGYALAGSS ANFEFKAGTD 
481:	TKNGTATFNN DISLGRFVNL KVDAHTANFK GIDTGNGGFN TLDFSGVTGK VNINKLITAS 
541:	TNVAVKNFNI NELVVKTNGV SVGEYTHFSE DIGSQSRINT VRLETGTRSI FSGGVKFKSG 
601:	EKLVIDEFYY SPWNYFDARN IKNVEITRKF ASSTPENPWG TSKLMFNNLT LGQNAVMDYS 
661:	QFSNLTIQGD FINNQGTINY LVRGGQVATL NVGNAAAMFF SNNVDSATGF YQPLMKINSA 
721:	QDLIKNKEHV LLKAKIIGYG NVSLGTNSIS NVNLIEQFKE RLALYNNNNR MDICVVRNTD 
781:	DIKACGTAIG NQSMVNNPDN YKYLIGKAWK NIGISKTANG SKISVYYLGN STPTEKGGNT 
841:	TNLPTNTTSN VRSANNALAQ NAPFAQPSAT PNLVAINQHD FGTIESVFEL ANRSKDIDTL 
901:	YANSGAQGRD LLQTLLIDSH DAGYARQMID NTSTGEITKQ LNAATTTLNN IASLEHKTSS 
961:	LQTLSLSNAM ILNSRLVNLS RRHTNNIDSF AQRLQALKDQ KFASLESAAE VLYQFAPKYE 
1021:	KPTNVWANAI GGTSLNNGGN ASLYGTSAGV DAYLNGEVEA IVGGFGSYGY SSFNNQANSL 
1081:	NSGANNTNFG VYSRIFANQH EFDFEAQGAL GSDQSSLNFK SALLRDLNQS YNYLAYSAAT 
1141:	RASYGYDFAF FRNALVLKPS VGVSYNHLGS TNFKSNSNQV ALKNGSSSQH LFNASANVEA 
1201:	RYYYGDTSYF YMNAGVLQEF ANFGSSNAVS LNTFKVNAAH NPLSTHARVM MGGELKLAKE 
1261:	VFLNLGFVYL HNLISNIGHF ASNLGMRYSF