TCDB is operated by the Saier Lab Bioinformatics Group
« See all members of the family


1.C.91.1.1
The tetrameric Stefin B pore-forming protein (98aas); structure known (20CT_A)

Accession Number:P04080
Protein Name:Cystatin-B
Length:98
Molecular Weight:11140.00
Species:Homo sapiens (Human) [9606]
Location1 / Topology2 / Orientation3: Cytoplasm1
Substrate Solutes

Cross database links:

Genevestigator: P04080
eggNOG: prNOG20772
HEGENOM: HBG715750
RefSeq: NP_000091.1   
Entrez Gene ID: 1476   
Pfam: PF00031   
OMIM: 254800  phenotype
601145  gene
KEGG: hsa:1476   

Gene Ontology

GO:0005737 C:cytoplasm
GO:0005730 C:nucleolus
GO:0004869 F:cysteine-type endopeptidase inhibitor activity
GO:0002020 F:protease binding
GO:0010466 P:negative regulation of peptidase activity

References (10)

[1] “Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver.”  Ritonja A.et.al.   3902020
[2] “Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1).”  Pennacchio L.A.et.al.   8596935
[3] “The DNA sequence of human chromosome 21.”  Hattori M.et.al.   10830953
[4] “The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).”  The MGC Project Teamet.al.   15489334
[5] “Nuclear localization of cystatin B, the cathepsin inhibitor implicated in myoclonus epilepsy (EPM1).”  Riccio M.et.al.   11139332
[6] “Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.”  Gauci S.et.al.   19413330
[7] “An extensive survey of tyrosine phosphorylation revealing new sites in human mammary epithelial cells.”  Heibeck T.H.et.al.   19534553
[8] “Lysine acetylation targets protein complexes and co-regulates major cellular functions.”  Choudhary C.et.al.   19608861
[9] “The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with the cysteine proteinase papain: a novel type of proteinase inhibitor interaction.”  Stubbs M.T.et.al.   2347312
[10] “Identification of mutations in cystatin B, the gene responsible for the Unverricht-Lundborg type of progressive myoclonus epilepsy (EPM1).”  Lalioti M.D.et.al.   9012407
Structure:
1STF   2OCT   4N6V     

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
Window Size: Angle:  
FASTA formatted sequence
1:	MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG 
61:	DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF