1.E.11 The
See 1.E for a generalized description of Holins.
The pneumococcal EJ-1 phage holin (EJh) is a hydrophobic peptide (85 aas) with 2 putative TMSs displaying lethal inner membrane disruptive activity. The synthetic N-terminal TMS flanked by its N-terminal positively charged residues folds into a transmembrane
This family belongs to the: Holin Superfamily I.
References associated with 1.E.11 family:
Bon, J., N. Mani, and R.K. Jayaswal. (1997). Molecular analysis of lytic genes of bacteriophage 80 alpha of Staphylococcus aureus. Can. J. Microbiol. 43: 612-616. 9246739
Diaz, E., M. Munthali, H. Lunsdorf, J.V. Holtje, and K.N. Timmis. (1996). The two-step lysis system of pneumococcal bacteriophage EJ-1 is functional in gram-negative bacteria: triggering of the major pneumococcal autolysin in Escherichia coli. Mol. Microbiol. 19: 667-681. 8820638
Haro, A., M. Vélez, E. Goormaghtigh, S. Lago, J. Vázquez, D. Andreu, and M. Gasset. (2003). Reconstitution of holin activity with a synthetic peptide containing the 1-32 sequence region of EJh, the EJ-1 phage holin. J. Biol. Chem. 278: 3929-3936. 12464609
Loessner, M.J., S. Gaeng, G. Wendlinger, S.K. Maier, and S. Scherer. (1998). The two-component lysis system of Staphylococcus aureus bacteriophage Twort: a large TTG-start holin and an associated amidase endolysin. FEMS Microbiol. Lett. 162: 265-274. 9627962
Seal, B.S., D.E. Fouts, M. Simmons, J.K. Garrish, R.L. Kuntz, R. Woolsey, K.M. Schegg, A.M. Kropinski, H.W. Ackermann, and G.R. Siragusa. (2011). Clostridium perfringens bacteriophages ΦCP39O and ΦCP26F: genomic organization and proteomic analysis of the virions. Arch Virol 156: 25-35. 20963614
