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1.E.2.1.1
Lysis protein S of phage lambda, holin S105.  The lambda-holin protein can mediate a caspase-independent non-apoptotic mode of cell death (Agu et al. 2007).  Topological class I with three TMSs.  The N-terminus is outside and the C-terminus is inside (Graschopf and Bläsi 1999; Gründling et al. 2000).  Nearly identical to the holin of Shigella phage Sf6 (Dover et al. 2016).

Accession Number:P03705
Protein Name:VLYS aka S
Length:107
Molecular Weight:11520.00
Species:Bacteriophage lambda [10710]
Number of TMSs:2
Location1 / Topology2 / Orientation3: Host cell inner membrane1 / Multi-pass membrane protein2
Substrate endolysin

Cross database links:

RefSeq: NP_040644.1    YP_001551775.1   
Entrez Gene ID: 2703479    5740919   
Pfam: PF05106   

Gene Ontology

GO:0020002 C:host cell plasma membrane
GO:0016021 C:integral to membrane
GO:0016998 P:cell wall macromolecule catabolic process
GO:0019835 P:cytolysis

References (2)

[1] “Nucleotide sequence of bacteriophage lambda DNA.”  Sanger F.et.al.   6221115
[2] “Mutational analysis of bacteriophage lambda lysis gene S.”  Raab R.et.al.   2943725

External Searches:

  • Search: DB with
  • BLAST ExPASy (Swiss Institute of Bioinformatics (SIB) BLAST)
  • CDD Search (Conserved Domain Database)
  • Search COGs (Clusters of Orthologous Groups of proteins)
  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
Window Size: Angle:  
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FASTA formatted sequence
1:	MKMPEKHDLL AAILAAKEQG IGAILAFAMA YLRGRYNGGA FTKTVIDATM CAIIAWFIRD 
61:	LLDFAGLSSN LAYITSVFIG YIGTDSIGSL IKRFAAKKAG VEDGRNQ