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1.E.21.2.1
Holin, hol118 of 96 aas and 3 TMSs (Kuo et al. 2009).  A stably engineered holin-expressing suicidal attenuated strain of Listeria monocytogenes has been used to deliver proteins and DNA to mammalian intestinal epithelial cells (Kuo et al. 2009).

Accession Number:Q37975
Protein Name:hol118
Length:96
Molecular Weight:10301.00
Species:Bacteriophage A118 [40521]
Number of TMSs:3
Location1 / Topology2 / Orientation3: Host membrane1 / Multi-pass membrane protein2
Substrate autolysin, small molecules

Cross database links:

RefSeq: NP_463485.1   
Entrez Gene ID: 922401   
Pfam: PF06946   

Gene Ontology

GO:0033644 C:host cell membrane
GO:0016021 C:integral to membrane
GO:0016998 P:cell wall macromolecule catabolic process
GO:0019835 P:cytolysis

References (2)

[1] “Heterogeneous endolysins in Listeria monocytogenes bacteriophages: a new class of enzymes and evidence for conserved holin genes within the siphoviral lysis cassettes.”  Loessner M.J.et.al.   8577256
[2] “Complete nucleotide sequence, molecular analysis and genome structure of bacteriophage A118 of Listeria monocytogenes: implications for phage evolution.”  Loessner M.J.et.al.   10652093

External Searches:

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  • 2° Structure (Network Protein Sequence Analysis)

Analyze:

Predict TMSs (Predict number of transmembrane segments)
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FASTA formatted sequence
1:	MIEMEFGKEL LVYMTFLVVV TPVFVQAIKK TELVPSKWLP TVSILIGAIL GALATFLDGS 
61:	GSLATMIWAG ALAGAGGTGL FEQFTNRSKK YGEDDK